BioBricks

All our parts are in the pSB1C3 backbone vector (abbreviate C3). When you click on the part's name, you are redirected to the partsregistry corresponding page.

First the legend:

• Ori = Origin of replication for Asaia and E.Coli
• S = Strong Promoter
• W = Weak Promoter
• Immu = immunotoxin
• P25 = Pfs25 protein
• P28 = Pfs28 protein
• Kan = Resistance to Kanamycin
• Tet = Resistance to Tetracycline

We made basic parts to build the complexest one.

[http://partsregistry.org/Part:BBa_K320000 *C3 + Ori] We made this plasmid because we needed an origin of replication that works in Asaia. After experiences we conclued that it also work with E.Coli

[http://partsregistry.org/Part:BBa_K320001 *C3 + Immu] The immunotoxine is the main part of the project, we obtain it by syntetisation. For a better unerstanding, go there.

[http://partsregistry.org/Part:BBa_K320002 *C3 + S] We made a new strong promoter permitting use to test teh expression of the immunotoxine. We called it strong because we used the constitutive sequence which should give the biggets level of expression.

[http://partsregistry.org/Part:BBa_K320010 *C3 + W] This promoter has been made to have a second level of expression for our proteins, which is lower than the strong one.

We made the following BioBricks from the aboves :

Building the biobricks...

[http://partsregistry.org/Part:BBa_K320003 *C3 + Ori + Tet]

[http://partsregistry.org/Part:BBa_K320004 *C3 + Ori + Kan]

[http://partsregistry.org/Part:BBa_K320005 *C3 + S + CFP]

[http://partsregistry.org/Part:BBa_K320006 *C3 + S + Immu]

[http://partsregistry.org/Part:BBa_K320007 *C3 + S + P25,]

[http://partsregistry.org/Part:BBa_K320008 *C3 + S + Immu + ori + Kan]

[http://partsregistry.org/Part:BBa_K320009 *C3 + S + P28 + Ori + Tet]

[http://partsregistry.org/Part:BBa_K320011 *C3 + Ori + Amp]

[http://partsregistry.org/Part:BBa_K320012 *C3 + W + Immu]

[http://partsregistry.org/Part:BBa_K320013 *C3 + W + P25]