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Revision as of 07:56, 28 June 2010 (view source) Cyu286 (Talk | contribs) ← Older edit		Revision as of 19:53, 28 June 2010 (view source) Gcxmatt (Talk | contribs) (→Assembly) Newer edit →
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	===[[Ligations]]===		===[[Ligations]]===
	===[[Mini Prep]]===		===[[Mini Prep]]===
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		+	=='''Microscopy'''==

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Revision as of 19:53, 28 June 2010

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Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 Assembly 3.1 Restriction Enzyme Digests 3.2 3A Assembly 3.3 Ligations 3.4 Mini Prep 4 Microscopy

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy