Team:EPF Lausanne/Project parts

From 2010.igem.org

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= BioBricks =
= BioBricks =
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All our parts are in the pSB1C3 backbone vector (abbreviate C3). When you click on the part's name, you are redirected to the partsregistry corresponding page.
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For the moment we have 14 BioBricks verified by sequencing and by electophoresis.
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We made basic parts to build the complexest one.  
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Those BioBricks are in the vector pSB1C3 (friendly called C3) and are ready to be send to the registry!
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The BioBricks we have for the moment (click on the name to go to the registry):
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[http://partsregistry.org/Part:BBa_K320000 *C3 + Ori]
[http://partsregistry.org/Part:BBa_K320000 *C3 + Ori]
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[http://partsregistry.org/Part:BBa_K320002 *C3 + S]
[http://partsregistry.org/Part:BBa_K320002 *C3 + S]
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[http://partsregistry.org/Part:BBa_K320010 *C3 + W]
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We made the following BioBricks from the aboves :
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[[Image:Asaia_Parts.png|300px|thumb|right|Building the biobricks...]]
[[Image:Asaia_Parts.png|300px|thumb|right|Building the biobricks...]]
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[http://partsregistry.org/Part:BBa_K320009 *C3 + S + P28 + Ori + Tet]
[http://partsregistry.org/Part:BBa_K320009 *C3 + S + P28 + Ori + Tet]
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[http://partsregistry.org/Part:BBa_K320010 *C3 + W]
 
[http://partsregistry.org/Part:BBa_K320011 *C3 + Ori + Amp]
[http://partsregistry.org/Part:BBa_K320011 *C3 + Ori + Amp]

Revision as of 20:32, 24 October 2010



BioBricks

All our parts are in the pSB1C3 backbone vector (abbreviate C3). When you click on the part's name, you are redirected to the partsregistry corresponding page.

We made basic parts to build the complexest one.

[http://partsregistry.org/Part:BBa_K320000 *C3 + Ori]

[http://partsregistry.org/Part:BBa_K320001 *C3 + Immu]

[http://partsregistry.org/Part:BBa_K320002 *C3 + S]

[http://partsregistry.org/Part:BBa_K320010 *C3 + W]


We made the following BioBricks from the aboves :

Building the biobricks...

[http://partsregistry.org/Part:BBa_K320003 *C3 + Ori + Tet]

[http://partsregistry.org/Part:BBa_K320004 *C3 + Ori + Kan]

[http://partsregistry.org/Part:BBa_K320005 *C3 + S + CFP]

[http://partsregistry.org/Part:BBa_K320006 *C3 + S + Immuno ]

[http://partsregistry.org/Part:BBa_K320007 *C3 + S + P25 ]

[http://partsregistry.org/Part:BBa_K320008 *C3 + S + immuno + ori + Kan]

[http://partsregistry.org/Part:BBa_K320009 *C3 + S + P28 + Ori + Tet]


[http://partsregistry.org/Part:BBa_K320011 *C3 + Ori + Amp]

[http://partsregistry.org/Part:BBa_K320012 *C3 + W + Immun]

[http://partsregistry.org/Part:BBa_K320013 *C3 + W + P25]


Legend:

  • Ori = Origin of replication for Asaia and E.Coli
  • S = Strong Promoter
  • W = Weak Promoter
  • Immu = immunotoxin
  • P25 = P25 protein
  • P28 = P28 protein
  • Kan = Resistance to Kanamycin
  • Tet = Resistance to Tetracycline





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