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Team:Cambridge/Tools/Gibson
From 2010.igem.org
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<html><div style="float:left; padding:0px 10px;"><img src="http://www.gibthon.org/images/gibthon.png" style="height:200px;" /></div><div style="height:180px;"><h3>Gibthon Construct Designer</h3> is a software tool under active development to aid with the design of primers for Gibson Assembly. It currently comes in two flavours - Gibthon Beta, and Gibthon Construct Designer Beta. The software is released under the <a href="http://www.gibthon.org/LICENSE">BSD license</a>, and the source code can be obtained for either by <a href="mailto:source@gibthon.org?subject="Gibthon%20%Source%20Code">email</a></div> | <html><div style="float:left; padding:0px 10px;"><img src="http://www.gibthon.org/images/gibthon.png" style="height:200px;" /></div><div style="height:180px;"><h3>Gibthon Construct Designer</h3> is a software tool under active development to aid with the design of primers for Gibson Assembly. It currently comes in two flavours - Gibthon Beta, and Gibthon Construct Designer Beta. The software is released under the <a href="http://www.gibthon.org/LICENSE">BSD license</a>, and the source code can be obtained for either by <a href="mailto:source@gibthon.org?subject="Gibthon%20%Source%20Code">email</a></div> | ||
| - | <h4>Gibthon Beta - <a href="http:// | + | <h4>Gibthon Beta - <a href="http://old.gibthon.org/">here</a></h4></html> |
{{:Team:Cambridge/Templates/rightpic|src=Gibthon-beta.png}}<html> | {{:Team:Cambridge/Templates/rightpic|src=Gibthon-beta.png}}<html> | ||
The more basic of the two tools, this will take as an input the sequences surrounding the join for which you wish to design primers. You can interactively adjust the length of the primers to tune the melting temperature of the DNA and check for secondary structures.<br /><br /> | The more basic of the two tools, this will take as an input the sequences surrounding the join for which you wish to design primers. You can interactively adjust the length of the primers to tune the melting temperature of the DNA and check for secondary structures.<br /><br /> | ||
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The developer is currently focussing on GCD, but will still be supporting Gibthon - contact him <a href="mailto:info@gibthon.org?subject=Gibthon%20Beta">here</a> | The developer is currently focussing on GCD, but will still be supporting Gibthon - contact him <a href="mailto:info@gibthon.org?subject=Gibthon%20Beta">here</a> | ||
| - | <h4>Gibthon Construct Designer Beta - <a href="http:// | + | <h4>Gibthon Construct Designer Beta - <a href="http://www.gibthon.org">here</a></h4></html> |
{{:Team:Cambridge/Templates/rightpic|src=Gibthon-dc-beta.png}}<html> | {{:Team:Cambridge/Templates/rightpic|src=Gibthon-dc-beta.png}}<html> | ||
Gibthon's older brother, and much more powerful. With this, you can design your construct from its component genes and automatically generate the optimal primers. The tool has access to the Parts Registry Database, the NCBI's Nucleotide database, as well as accepting genbank formatted files from the user. The user is able to store constructs and their own genes for recovery later.<br /><br /> | Gibthon's older brother, and much more powerful. With this, you can design your construct from its component genes and automatically generate the optimal primers. The tool has access to the Parts Registry Database, the NCBI's Nucleotide database, as well as accepting genbank formatted files from the user. The user is able to store constructs and their own genes for recovery later.<br /><br /> | ||
Revision as of 20:22, 24 October 2010
Tools: Gibthon
Gibthon Construct Designer
is a software tool under active development to aid with the design of primers for Gibson Assembly. It currently comes in two flavours - Gibthon Beta, and Gibthon Construct Designer Beta. The software is released under the BSD license, and the source code can be obtained for either by emailGibthon Beta - here
The developer is currently focussing on GCD, but will still be supporting Gibthon - contact him here
Gibthon Construct Designer Beta - here
Generating the primers for PCR extension takes only a few seconds each, and the information is returned to the user in the form of a PDF with a datasheet for each primer. In the near future it will also provide all the necessary protocols to complete your construct. As time goes by, more granular conrol over settings will become available.
The algorithm for optimising primer length is still under development, so should not be entirely trusted until the software comes out of beta. When you find something that doesn't work, or could be improved, please email the developer.
How it works
This is likely to be changing on a fairly rapid basis - for the latest info, just visit the site
