Team:EPF Lausanne/Notebook/week10

From 2010.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 49: Line 49:
*Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).  
*Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).  
-
| style="width: 50%" valign="top" |
+
| style="width: 30%" valign="top" |
== Other ==
== Other ==
*Competence of E.Coli DB3.1
*Competence of E.Coli DB3.1
-
| style="width: 50%" valign="top" |
+
| style="width: 20%" valign="top" |
== Movie ==
== Movie ==
*Continued working on movie.  
*Continued working on movie.  
Line 62: Line 62:
= 14-09-2010 =
= 14-09-2010 =
{| cellspacing="15"
{| cellspacing="15"
-
| style="width: 33.33%" valign="top" |
+
| style="width: 50%" valign="top" |
== Cloning ==
== Cloning ==
-
*Western Blot from yesterday finished. Observed one band which did NOT correspond to the right size. Furthermore the negative control failed! Reason not known. Maybe it is another protein with a His-tag which is present on the C3 plasmid.  
+
 
 +
*Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.  
-
| style="width: 33.33%" valign="top" |
+
| style="width: 50%" valign="top" |
-
 
+
-
== Drosophila ==
+
-
| style="width: 33.33%" valign="top" |
 
== Other ==
== Other ==
-
*Gwen made us a chocolate cake!! Yeah!
 
-
| style="width: 33.33%" valign="top" |
 
|}
|}
= 15-09-2010 =
= 15-09-2010 =
{| cellspacing="15"
{| cellspacing="15"
-
| style="width: 33.33%" valign="top" |
+
| style="width: 50%" valign="top" |
== Cloning ==
== Cloning ==
-
*Western Blot from yesterday repeated. Same result as yesterday.  
+
 
 +
*Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!
-
| style="width: 33.33%" valign="top" |
+
| style="width: 50%" valign="top" |
-
== Drosophila ==
 
-
 
-
| style="width: 33.33%" valign="top" |
 
== Other ==
== Other ==
-
*Solange has a hangover! ;)
+
 
|}
|}
Line 99: Line 93:
| style="width: 50%" valign="top" |
| style="width: 50%" valign="top" |
-
== Movie ==
+
== Other ==
-
*Started making the stop motion movie.
+
 
|}
|}
= 17-09-2010 =
= 17-09-2010 =
Line 109: Line 103:
| style="width: 50%" valign="top" |
| style="width: 50%" valign="top" |
-
== Movie ==
+
== Other ==
-
*Continued working on movie.
+
|}
|}
Line 129: Line 122:
 +
{{EPFL_2010_bottom_wrap}}
</div>
</div>

Latest revision as of 19:48, 24 October 2010









Contents

Week 10

13-09-2010

Cloning

  • Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Other

  • Competence of E.Coli DB3.1


Movie

  • Continued working on movie.

14-09-2010

Cloning

  • Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.

Other

15-09-2010

Cloning

  • Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!

Other

16-09-2010

Cloning

Other

17-09-2010

Cloning

Other









wrap bottom

Retrieved from "http://2010.igem.org/Team:EPF_Lausanne/Notebook/week10"