Team:Yale/Our Project/Protocols/TSI agar

From 2010.igem.org

(Difference between revisions)
(New page: <br /> The composition of the plates were:<br /> <br /><li>500 mL water</a></li> <li>1.5 g Meat Extract </a></li> <li>1.5 g Yeast Extract</a></li> <li>7.5 g Pancreatic Digest of Casein<...)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
-
<br /> The composition of the plates were:<br />
+
__NOTOC__
 +
{{:Team:Yale/Templates/Yale_Header_Project}}
 +
<html>
 +
<body>
 +
<div id="main-content">
 +
<div id="left-col">
 +
<h5>
 +
our project
 +
</h5>
 +
<p>
 +
<ul id="proj-nav">
 +
<li><a href="https://2010.igem.org/Team:Yale/Our Project">introduction</a></li>
 +
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li>
 +
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></li>
 +
<li><b><a href="https://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></b></li>
 +
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
 +
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Future Work">future work</a></li>
 +
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Applications">applications</a></li>
 +
</ul>
 +
</p>
 +
</div>
-
<br /><li>500 mL water</a></li>
+
<div id="right-col">
-
<li>1.5 g Meat Extract </a></li>
+
<h5>
-
<li>1.5 g Yeast Extract</a></li>
+
protocols
-
<li>7.5 g Pancreatic Digest of Casein</a></li>
+
</h5>
-
<li> 2.5 g Protease Peptone</a></li>
+
<p>
-
<li>0.5 g Dextrose</a></li>
+
<!------------- WRITE HERE------------->
-
<li>0.5 g Lactose</a></li>
+
<h4> Triple Sugar Iron (TSI) Agar Recipe </h4>
-
<li>0.5 g Sucrose</a></li>
+
<ul>
-
<li>0.26 g Ferrous Ammonium Sulfate (hexahydrate)</a></li>
+
 
-
<li>2.5 g NaCl</a></li>
+
<li>500 mL water </li>  
-
<li>0.15 g Sodium Thiosulfate</a></li>
+
<li>1.5 g Meat Extract </li>
-
<li>6 g Agar</a></li>
+
<li>1.5 g Yeast Extract</li>
-
<li>12 mg Phenol Red</a></li>
+
<li>7.5 g Pancreatic Digest of Casein</li>
-
<li>+ Ampicillin</a></li>
+
<li> 2.5 g Protease Peptone</li>
 +
<li>0.5 g Dextrose</li>
 +
<li>0.5 g Lactose</li>
 +
<li>0.5 g Sucrose</li>
 +
<li>0.26 g Ferrous Ammonium Sulfate (hexahydrate)</li>
 +
<li>2.5 g NaCl</li>
 +
<li>0.15 g Sodium Thiosulfate</li>
 +
<li>6 g Agar</li>
 +
<li>12 mg Phenol Red</li>
 +
<li>+ Ampicillin</li>
 +
</br>
 +
 
 +
</ul>
 +
<!------------- BUT NOT BELOW HERE ------------->
 +
</p>
 +
</div>
 +
 
 +
 
 +
</div>
 +
</body>
 +
</html>

Latest revision as of 19:35, 24 October 2010

iGEM Yale

protocols

Triple Sugar Iron (TSI) Agar Recipe

  • 500 mL water
  • 1.5 g Meat Extract
  • 1.5 g Yeast Extract
  • 7.5 g Pancreatic Digest of Casein
  • 2.5 g Protease Peptone
  • 0.5 g Dextrose
  • 0.5 g Lactose
  • 0.5 g Sucrose
  • 0.26 g Ferrous Ammonium Sulfate (hexahydrate)
  • 2.5 g NaCl
  • 0.15 g Sodium Thiosulfate
  • 6 g Agar
  • 12 mg Phenol Red
  • + Ampicillin

Retrieved from "http://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar"