Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/23

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/09/23=
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===1:DNA Digestion===
===1:DNA Digestion===
<Member><br />
<Member><br />
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<Sample, Materials><br />
<Sample, Materials><br />
・PCR productions
・PCR productions
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*1
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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*4
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
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*5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===2:PCR===
===2:PCR===
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<Sample>(temperature in annealing)<br />
<Sample>(temperature in annealing)<br />
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*7(67.5)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7](67.5)
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*9(57,61,69)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9](57,61,69)
<Protocol><br />
<Protocol><br />
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<Sample><br />
<Sample><br />
Digestion products.
Digestion products.
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*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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*3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
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*4
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
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*5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
<Protocol><br />
<Protocol><br />
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
 
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[[Image:]]
 
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===6:DNA extraction from gels===
===6:DNA extraction from gels===
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<Sample, Materials><br />
<Sample, Materials><br />
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*2 (9/23)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/23)
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*4 (9/23)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/23)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]

Latest revision as of 18:17, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/09/23

1:DNA Digestion

<Member>
Hitomi

<Sample, Materials>
・PCR productions

<Protocol>
See Prptpcpl 9

2:PCR

<Member>
Hitomi

<Sample>(temperature in annealing)

  • 7(67.5)
  • 9(57,61,69)

<Protocol>
See Protocol 1

3:DNA extraction

<Member>
Hitomi

<Sample>
PCR products(9/23)

<Protocol>
See Protocol 4


4:Electrophoresis

<Member>
Hitomi

<Sample>
Digestion products.

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-23 20hr 35min.JPG

5:Electrophoresis

<Member>
Hitomi

<Sample>
PCR products (9/23)

<Protocol>
See Protocol 8

6:DNA extraction from gels

<Member>
Mariko,Hitomi

<Sample>
Digestion products (9/23) (After electrophoresis)

<Protocol>
See Protocol 4


7:DNA Digestion

<Member>
Hitomi

<Sample, Materials>

  • 2 (9/23)
  • 4 (9/23)

<Protocol>
See Prptpcpl 9