Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08

From 2010.igem.org

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<Sample><brt />
<Sample><brt />
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*5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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*1+2(ligation 2010/08/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2](ligation 2010/08/31)
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*4+5(ligation 2010/08/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5](ligation 2010/08/31)
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*7+8(ligation 2010/09/06)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7+8](ligation 2010/09/06)
<Protocol><br />
<Protocol><br />
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*Tube(temperature in annealing)
*Tube(temperature in annealing)
1.(71.0,70.5,70.0℃)<br />
1.(71.0,70.5,70.0℃)<br />
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2.(72.0,71.5℃)
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2.(72.0,71.5℃)<br />
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3.(70.0℃)
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3.(70.0℃)<br />
4.(70.0℃)
4.(70.0℃)
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<Sample><br />
<Sample><br />
PCR products.
PCR products.
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:*5H
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H]
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:*5M
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5M]
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:*5L
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5L]
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:*1-2H
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2H]
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:*1-2L
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2L]
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:*4-5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
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:*7-8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result>
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<Result><br />
[[Image:Matsuura_2010-08-06_20hr_02min_(17).JPG]]
[[Image:Matsuura_2010-08-06_20hr_02min_(17).JPG]]

Latest revision as of 18:14, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
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22 23 24 25 26 27 28
29 30 31
September 2010
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19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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10 11 12 13 14 15 16
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31

2010/09/08

1:PCR

<Member>
Hitomi

<Sample><brt />

  • 5
  • 1+2(ligation 2010/08/31)
  • 4+5(ligation 2010/08/31)
  • 7+8(ligation 2010/09/06)

<Protocol>
See Protocol 2

  • Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)
2.(72.0,71.5℃)
3.(70.0℃)
4.(70.0℃)

Total 12 tubes.

2:Electrophoresis

<Member>
mizuki, Hitomi

<Sample>
PCR products.

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-08-06 20hr 02min (17).JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/08"