Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08

From 2010.igem.org

(Difference between revisions)
(→PCR)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
 +
=2010/09/08=
=2010/09/08=
-
===PCR===
 
-
<Member>
 
 +
===1:PCR===
 +
<Member><br />
Hitomi
Hitomi
 +
<Sample><brt />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2](ligation 2010/08/31)
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5](ligation 2010/08/31)
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7+8](ligation 2010/09/06)
-
<Sample>
+
<Protocol><br />
-
 
+
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
-
*5
+
-
*1+2(ligation 2010/08/31)
+
-
*4+5(ligation 2010/08/31)
+
-
*7+8(ligation 2010/09/06)
+
-
 
+
-
 
+
-
<Protocol>
+
-
 
+
-
See Protocol2
+
-
(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
+
-
 
+
*Tube(temperature in annealing)
*Tube(temperature in annealing)
-
 
+
1.(71.0,70.5,70.0℃)<br />
-
:1.(71.0,70.5,70.0℃)
+
2.(72.0,71.5℃)<br />
-
:2.(72.0,71.5℃)
+
3.(70.0℃)<br />
-
:3.(70.0℃)
+
4.(70.0℃)
-
:4.(70.0℃)
+
:Total 12 tubes.
:Total 12 tubes.
-
 
+
===2:Electrophoresis===
-
===Electrophoresis===
+
<Member><br />
-
 
+
-
<Member>
+
mizuki, Hitomi
mizuki, Hitomi
 +
<Sample><br />
 +
PCR products.
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5M]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5L]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2H]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2L]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8]
-
<Sample>
+
<Protocol><br />
-
 
+
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
+
-
 
+
-
<Protocol>
+
-
 
+
-
 
+
-
 
+
-
<Result>
+
-
[[Image:Matsuura_2010-09-08_18hr_09min.tif]]
+
<Result><br />
 +
[[Image:Matsuura_2010-08-06_20hr_02min_(17).JPG]]

Latest revision as of 18:14, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/08

1:PCR

<Member>
Hitomi

<Sample><brt />

  • 5
  • 1+2(ligation 2010/08/31)
  • 4+5(ligation 2010/08/31)
  • 7+8(ligation 2010/09/06)

<Protocol>
See Protocol 2

  • Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)
2.(72.0,71.5℃)
3.(70.0℃)
4.(70.0℃)

Total 12 tubes.

2:Electrophoresis

<Member>
mizuki, Hitomi

<Sample>
PCR products.

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-08-06 20hr 02min (17).JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/08"