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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/30
From 2010.igem.org
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| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
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=2010/08/30= | =2010/08/30= | ||
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＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR productions | ・PCR productions | ||
| - | *1L(8/25) 8μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25) 8μl |
| - | *2L(8/26) 8μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26) 8μl |
| - | *4H(8/26) 16μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26) 16μl |
| - | *5H(8/26) 16μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26) 16μl |
・Digest enzyme | ・Digest enzyme | ||
| Line 49: | Line 50: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | ||
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==3：Electrophoresis== | ==3：Electrophoresis== | ||
| Line 73: | Line 73: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1L(8/25) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25) |
| - | *2L(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26) |
| - | *4H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26) |
| - | *5H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26) |
(after digestion) | (after digestion) | ||
| Line 87: | Line 87: | ||
＜Result＞<br /> | ＜Result＞<br /> | ||
| - | [[Image:Matsuura_2010-08- | + | [[Image:Matsuura_2010-08-06_20hr_02min_(8).JPG]] |
==5：DNA Ligation == | ==5：DNA Ligation == | ||
| Line 95: | Line 95: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1L(8/25) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25) |
| - | *2L(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26) |
| - | *4H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26) |
| - | *5H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26) |
(after digestion) | (after digestion) | ||
| Line 107: | Line 107: | ||
We ligated 1 and 2, 4 and 5. | We ligated 1 and 2, 4 and 5. | ||
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==6：Electrophoresis== | ==6：Electrophoresis== | ||
| Line 115: | Line 114: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1L(8/25) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25) |
| - | *2L(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26) |
| - | *4H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26) |
| - | *5H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26) |
(after ligation) | (after ligation) | ||
| Line 128: | Line 127: | ||
＜Result＞<br /> | ＜Result＞<br /> | ||
| - | [[Image:Matsuura_2010-08-06_20hr_02min_( | + | [[Image:Matsuura_2010-08-06_20hr_02min_(9).JPG]] |
Latest revision as of 18:12, 24 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/08/30
1：PCR
＜Member＞
Mariko, Hitomi, Taka
＜Sample＞
・E-coli (having BBa_I13521 plasmid)
・E-coli (having BBa_K208017 plasmid)
・E-coli JM109 (NIPPON GENE)
＜Protocol＞
See Protocol 2
・Tube (temperature in annealing)
1.Promoter~signal (72.0℃)
3. mRFP~Terminator (69.0)
4. Promoter (70.0)
5. RBS~signal (70.0)
7. Terminator (69.0/67.5/66.0)
8. CRP (72.5)
Total 8 tubes.
2：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- AvrⅡ
- NheⅠ
- SpeⅠ
＜Protocol＞
See Protocol 9
3：Electrophoresis
＜Member＞
Mariko, Taka
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
4：Electrophoresis
＜Member＞
nito
＜Sample＞
(after digestion)
＜Protocol＞
SeeProtocol 8
And cut off gels included DNA.
＜Result＞
.JPG)
5：DNA Ligation
＜Member＞
nito
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 3
We ligated 1 and 2, 4 and 5.
6：Electrophoresis
＜Member＞
nito
＜Sample＞
(after ligation)
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
