Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/30

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Header}} =2010/08/30= ==1：PCR == ＜Member＞<br /> Mariko, Hitomi, Taka ＜Sample＞<br /> ・E-coli (having BBa_I13521 plasmid) ・E-coli (having BBa_K20...)
 
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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/08/30=
=2010/08/30=
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＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
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*1L(8/25)  8μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)  8μl
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*2L(8/26)  8μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)  8μl
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*4H(8/26)  16μl
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)  16μl
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*5H(8/26)  16μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)  16μl
・Digest enzyme
・Digest enzyme
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]
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==3：Electrophoresis==
==3：Electrophoresis==
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＜Result＞<br />
＜Result＞<br />
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[[Image:Matsuura 2010-08-06 20hr 02min (7).JPG]]
==4：Electrophoresis==
==4：Electrophoresis==
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)   
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*2L(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
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*4H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
-
*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after digestion)
(after digestion)
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And cut off gels included DNA.
And cut off gels included DNA.
 +
＜Result＞<br />
 +
[[Image:Matsuura_2010-08-06_20hr_02min_(8).JPG]]
==5：DNA Ligation ==
==5：DNA Ligation ==
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)   
-
*2L(8/26)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
-
*4H(8/26)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
-
*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after digestion)
(after digestion)
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We ligated 1 and 2, 4 and 5.
We ligated 1 and 2, 4 and 5.
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==6：Electrophoresis==
==6：Electrophoresis==
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)   
-
*2L(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
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*4H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
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*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after ligation)
(after ligation)
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＜Result＞
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＜Result＞<br />
 +
[[Image:Matsuura_2010-08-06_20hr_02min_(9).JPG]]

Latest revision as of 18:12, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/08/30

1：PCR

＜Member＞
Mariko, Hitomi, Taka


＜Sample＞
・E-coli (having BBa_I13521 plasmid)

・E-coli (having BBa_K208017 plasmid)

・E-coli JM109 (NIPPON GENE)


＜Protocol＞
See Protocol 2

・Tube (temperature in annealing)
1.Promoter~signal (72.0℃)
3. mRFP~Terminator (69.0)
4. Promoter (70.0)
5. RBS~signal (70.0)
7. Terminator (69.0/67.5/66.0)
8. CRP (72.5)

Total 8 tubes.


2：DNA Digestion

＜Member＞
nito


＜Sample, Materials＞
・PCR productions

  • 1L(8/25) 8μl
  • 2L(8/26) 8μl
  • 4H(8/26) 16μl
  • 5H(8/26) 16μl

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


＜Protocol＞
See Protocol 9

3：Electrophoresis

＜Member＞
Mariko, Taka


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (7).JPG

4：Electrophoresis

＜Member＞
nito


＜Sample＞

(after digestion)


＜Protocol＞
SeeProtocol 8

And cut off gels included DNA.

＜Result＞
Matsuura 2010-08-06 20hr 02min (8).JPG

5：DNA Ligation

＜Member＞
nito


＜Sample＞

(after digestion)


＜Protocol＞
See Protocol 3

We ligated 1 and 2, 4 and 5.

6：Electrophoresis

＜Member＞
nito


＜Sample＞

(after ligation)


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (9).JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/08/30"