Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/30

From 2010.igem.org

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=2010/08/30=
=2010/08/30=
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＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1l](8/25)  8μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)  8μl
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*2L(8/26)  8μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)  8μl
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*4H(8/26)  16μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)  16μl
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*5H(8/26)  16μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)  16μl
・Digest enzyme
・Digest enzyme
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)   
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*2L(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
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*4H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
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*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after digestion)
(after digestion)
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)   
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*2L(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
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*4H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
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*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after digestion)
(after digestion)
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We ligated 1 and 2, 4 and 5.
We ligated 1 and 2, 4 and 5.
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==6：Electrophoresis==
==6：Electrophoresis==
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L](8/25)   
-
*2L(8/26)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
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*4H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
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*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after ligation)
(after ligation)

Latest revision as of 18:12, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/08/30

1：PCR

＜Member＞
Mariko, Hitomi, Taka


＜Sample＞
・E-coli (having BBa_I13521 plasmid)

・E-coli (having BBa_K208017 plasmid)

・E-coli JM109 (NIPPON GENE)


＜Protocol＞
See Protocol 2

・Tube (temperature in annealing)
1.Promoter~signal (72.0℃)
3. mRFP~Terminator (69.0)
4. Promoter (70.0)
5. RBS~signal (70.0)
7. Terminator (69.0/67.5/66.0)
8. CRP (72.5)

Total 8 tubes.


2：DNA Digestion

＜Member＞
nito


＜Sample, Materials＞
・PCR productions

  • 1L(8/25) 8μl
  • 2L(8/26) 8μl
  • 4H(8/26) 16μl
  • 5H(8/26) 16μl

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


＜Protocol＞
See Protocol 9

3：Electrophoresis

＜Member＞
Mariko, Taka


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (7).JPG

4：Electrophoresis

＜Member＞
nito


＜Sample＞

(after digestion)


＜Protocol＞
SeeProtocol 8

And cut off gels included DNA.

＜Result＞
Matsuura 2010-08-06 20hr 02min (8).JPG

5：DNA Ligation

＜Member＞
nito


＜Sample＞

(after digestion)


＜Protocol＞
See Protocol 3

We ligated 1 and 2, 4 and 5.

6：Electrophoresis

＜Member＞
nito


＜Sample＞

(after ligation)


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (9).JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/08/30"