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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test16settembre
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The following day cultures were diluted 1:100 in a final volume of 1 ml and let grow again for about five hours at +37°C, 220 rpm. Then cultures were centrifuged at 2000 rpm, 25°C, the supernatants were removed and the pellets were resuspended. Optical density (O.D.) of each cell culture was than measured with TECAN Infinte F200. Samples were diluted in order to obtain the same O.D. equal to 0,02. Then O.D. and green Fluorecence were measured. | The following day cultures were diluted 1:100 in a final volume of 1 ml and let grow again for about five hours at +37°C, 220 rpm. Then cultures were centrifuged at 2000 rpm, 25°C, the supernatants were removed and the pellets were resuspended. Optical density (O.D.) of each cell culture was than measured with TECAN Infinte F200. Samples were diluted in order to obtain the same O.D. equal to 0,02. Then O.D. and green Fluorecence were measured. | ||
| - | ==RESULTS | + | ==RESULTS== |
| + | Cultures resulted immediately induced because of the high concentration of homoserine-lactone that remained in the solutions. This prevented from calculating the ODstart. | ||
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| + | [[Image:UNIPV_Pavia_FluoVSODHC01609.png| 620px | center ]] | ||
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| + | [[Image:UNIPV_Pavia_FluoVSODCo1609.png| 620px | center ]] | ||
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| + | [[Image:UNIPV_Pavia_FluoVSODLC01609.png| 620px | center ]] | ||
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Revision as of 18:00, 24 October 2010
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