Team:Northwestern/References

From 2010.igem.org

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== '''Overall project''' ==
 
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Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
 
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in chitinase-KO strain e coli
 
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== Project Details ==
 
=== Lac Operon Control ===
=== Lac Operon Control ===
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chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
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====Chitin Staining (Calcofluor)====
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Does not stain E Coli - http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf
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Stains Biofilm Polysaccharides? - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6V73-3YKKH2J-4S-1&_cdi=5831&_user=965532&_pii=0043135494003399&_orig=search&_coverDate=08%2F31%2F1995&_sk=999709991&view=c&wchp=dGLbVzz-zSkzS&md5=fedcb287ed9c9dafca2dbb4f2bc053a4&ie=/sdarticle.pdf
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Inhibits Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/
=== Apoptosis ===
=== Apoptosis ===
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registry: http://partsregistry.org/Cell_death
registry: http://partsregistry.org/Cell_death
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==== Live/Dead Staining aka Viability/Cytotoxicity Assay
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DMAO(Live), EtD-III(Dead) - http://www.promokine.info/products/cell-analysis/cell-staining-reagents-related-products/, http://www.biotium.com/product/product_info/Protocol/30027.pdf
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=== Chitinase Knockout (may not be necessary) ===
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==Keio Collection==
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http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp
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=== ChiA(Chitinase) Knockout (may not be necessary) ===
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
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chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
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===ydgg/tqsA (AI2-Transporter) Knockout===
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KO -> ~7000x biofilm thickness, ~500x biomass - http://jb.asm.org/cgi/content/short/188/2/587

Revision as of 16:04, 28 June 2010

Tech Institute

Home Brainstorm Team Acknowledgements Project Side Project Parts Notebook Calendar Protocol Safety Links References Media Contact


Contents

Lac Operon Control

http://openwetware.org/wiki/IPTG

Chitin Synthesis

chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

Chitin Staining (Calcofluor)

Does not stain E Coli - http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf

Stains Biofilm Polysaccharides? - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6V73-3YKKH2J-4S-1&_cdi=5831&_user=965532&_pii=0043135494003399&_orig=search&_coverDate=08%2F31%2F1995&_sk=999709991&view=c&wchp=dGLbVzz-zSkzS&md5=fedcb287ed9c9dafca2dbb4f2bc053a4&ie=/sdarticle.pdf

Inhibits Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/

Apoptosis

registry: http://partsregistry.org/Cell_death

==== Live/Dead Staining aka Viability/Cytotoxicity Assay

DMAO(Live), EtD-III(Dead) - http://www.promokine.info/products/cell-analysis/cell-staining-reagents-related-products/, http://www.biotium.com/product/product_info/Protocol/30027.pdf


Keio Collection

http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp

ChiA(Chitinase) Knockout (may not be necessary)

summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

ydgg/tqsA (AI2-Transporter) Knockout

KO -> ~7000x biofilm thickness, ~500x biomass - http://jb.asm.org/cgi/content/short/188/2/587