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Team:LMU-Munich/Jump-or-die/Schedule/SequenzPCR3
From 2010.igem.org
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| - | == | + | ==Sequence PCR3== |
| + | |||
| + | === with forward primer (primer 3)=== | ||
| + | NNNNN | ||
| + | |||
| + | |||
| + | === with reverse primer (primer 6)=== | ||
| + | GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNN | ||
| + | NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | ||
| + | |||
| + | |||
| + | verified by sequencing of BBa_K368016 (PCR 3 in pSB1C3) | ||
| + | |||
| + | === with forward primer=== | ||
| + | NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTG | ||
| + | |||
| + | NTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCA | ||
| + | |||
| + | ATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGA | ||
| + | |||
| + | TAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATG | ||
| + | |||
| + | ANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATA | ||
| + | |||
| + | AGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGAT | ||
| + | |||
| + | CGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTN | ||
| + | |||
| + | ACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGC | ||
| + | |||
| + | AAANNN | ||
| + | |||
| + | === with reverse primer=== | ||
| + | NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCA | ||
| + | |||
| + | TAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCC | ||
| + | |||
| + | TTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGA | ||
| + | |||
| + | TGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGG | ||
| + | |||
| + | AATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGT | ||
| + | |||
| + | ACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATT | ||
| + | |||
| + | TCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAG | ||
| + | |||
| + | CTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTG | ||
| + | |||
| + | GAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTG | ||
| + | |||
| + | TACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAG | ||
| + | |||
| + | CTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAA | ||
| + | |||
| + | TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGT | ||
| + | |||
| + | AAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA | ||
| + | |||
| + | GTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANC | ||
| + | |||
| + | ACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTG | ||
| + | |||
| + | CTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAA | ||
| + | |||
| + | GAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCAT | ||
| + | |||
| + | CCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCC | ||
| + | |||
| + | TNGNNNACCNTTTCCNNNNNNAACTGNANN | ||
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Latest revision as of 17:23, 24 October 2010
NNNNN
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTG
NTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCA
ATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGA
TAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATG
ANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATA
AGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGAT
CGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTN
ACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGC
AAANNN
NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCA
TAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCC
TTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGA
TGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGG
AATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGT
ACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATT
TCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAG
CTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTG
GAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTG
TACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAG
CTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAA
TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGT
AAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA
GTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANC
ACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTG
CTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAA
GAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCAT
CCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCC
TNGNNNACCNTTTCCNNNNNNAACTGNANN
Contents
Sequence PCR3
with forward primer (primer 3)
with reverse primer (primer 6)
verified by sequencing of BBa_K368016 (PCR 3 in pSB1C3)
with forward primer
with reverse primer
