Team:EPF Lausanne/Project droso
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+ | ===Drosophila=== | ||
+ | In parallel, we did experiments to assess if Asaia was persistant in ''[https://2010.igem.org/Team:EPF_Lausanne/Project_droso Drosophila] melanogaster ''. In fact, the fly is a much easier organism to grow and work on, in opposition to mosquitoes which need to feed on animals to reproduce. | ||
+ | Our experiment was set in 3 steps: | ||
+ | *infection of the flies with GFP-Asaia*, and 3 other bacteria as controls | ||
+ | *retrieval of the flies after 3h (control of infection), 24h, 48h | ||
+ | *counting of the number of colonies for all time points and the 4 differents strains | ||
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+ | The goal was to observe the persistance of Asaia in the Drosophila gut to see if it can be used as a model organism to study Asaia. | ||
+ | |||
+ | The results were negative for a the experience and we repeteated it 4 times. | ||
+ | |||
+ | ''graphe des drosophiles'' | ||
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Revision as of 17:11, 24 October 2010
![](https://static.igem.org/mediawiki/igem.org/c/c7/EPFL_Title_Background.png)
![](https://static.igem.org/mediawiki/2010/9/9c/EPFL_Title_2_Droso.png)
Drosophila
In parallel, we did experiments to assess if Asaia was persistant in Drosophila melanogaster . In fact, the fly is a much easier organism to grow and work on, in opposition to mosquitoes which need to feed on animals to reproduce. Our experiment was set in 3 steps:
- infection of the flies with GFP-Asaia*, and 3 other bacteria as controls
- retrieval of the flies after 3h (control of infection), 24h, 48h
- counting of the number of colonies for all time points and the 4 differents strains
The goal was to observe the persistance of Asaia in the Drosophila gut to see if it can be used as a model organism to study Asaia.
The results were negative for a the experience and we repeteated it 4 times.
graphe des drosophiles