Team:UNIPV-Pavia/Team/WwdPavia
From 2010.igem.org
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and performed with '''Federica''' the validation experiments on it. | and performed with '''Federica''' the validation experiments on it. | ||
Finally, he performed fluorescence assays on intein fusion proteins. | Finally, he performed fluorescence assays on intein fusion proteins. | ||
+ | |||
'''Alessandro Ranieri''' worked on: the design of phasin and intein fusion | '''Alessandro Ranieri''' worked on: the design of phasin and intein fusion | ||
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purification. He transferred the selected BioBrick parts in pSB1C3 | purification. He transferred the selected BioBrick parts in pSB1C3 | ||
shipping vector and performed the quality control on them. | shipping vector and performed the quality control on them. | ||
+ | |||
'''Federica Sampietro''' worked on: all the activities connected with | '''Federica Sampietro''' worked on: all the activities connected with | ||
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granules production (with '''Sara''') and the validation experiments on | granules production (with '''Sara''') and the validation experiments on | ||
phasin binding (with '''Manuel Z.'''). | phasin binding (with '''Manuel Z.'''). | ||
+ | |||
'''Chiara Tassorelli''' performed: an accurate analysis of past iGEM | '''Chiara Tassorelli''' performed: an accurate analysis of past iGEM | ||
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now the responsible of the poster and presentation design. She also | now the responsible of the poster and presentation design. She also | ||
designed the logo and worked on wiki design. | designed the logo and worked on wiki design. | ||
+ | |||
'''Manuel Zocco''' worked on: the construction of phasin fusion proteins | '''Manuel Zocco''' worked on: the construction of phasin fusion proteins | ||
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by PCR on the integrative vector for E. coli and he had the idea (with | by PCR on the integrative vector for E. coli and he had the idea (with | ||
'''Manuel L.''') of the integrative vector for E. coli. | '''Manuel L.''') of the integrative vector for E. coli. | ||
+ | |||
'''Manuel Lupotto''' had the idea of the integrative vector for E. coli (with | '''Manuel Lupotto''' had the idea of the integrative vector for E. coli (with | ||
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experiments about PHB granules production validation and the yeast integrative | experiments about PHB granules production validation and the yeast integrative | ||
vector validation (with '''Susanna'''). Primer design supervisor. | vector validation (with '''Susanna'''). Primer design supervisor. | ||
+ | |||
'''Matteo Meroso''' supervised all the activities | '''Matteo Meroso''' supervised all the activities | ||
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integrative vector for E. coli. He also supervised (with '''Susanna''') the | integrative vector for E. coli. He also supervised (with '''Susanna''') the | ||
design of experiments for the self-inducible devices. | design of experiments for the self-inducible devices. | ||
+ | |||
'''Lorenzo Pasotti''' coordinated the team during the brainstorming | '''Lorenzo Pasotti''' coordinated the team during the brainstorming | ||
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Synthetic Biology. He had the idea (with '''Susanna''' and '''Nicolò''') of the design of | Synthetic Biology. He had the idea (with '''Susanna''' and '''Nicolò''') of the design of | ||
self-inducible devices. | self-inducible devices. | ||
+ | |||
'''Giacomo Zambianchi''' supervised the design and construction of phasin | '''Giacomo Zambianchi''' supervised the design and construction of phasin | ||
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design, management and updating. He is also the freezer management | design, management and updating. He is also the freezer management | ||
coordinator and the advisor of the fundraising activities. | coordinator and the advisor of the fundraising activities. | ||
+ | |||
'''Susanna Zucca''' planned and coordinated all the student and advisor | '''Susanna Zucca''' planned and coordinated all the student and advisor | ||
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intein sub-projects. Instructor for the rest of the team about | intein sub-projects. Instructor for the rest of the team about | ||
promoter measurement, modeling and data analysis. | promoter measurement, modeling and data analysis. | ||
+ | |||
'''Gabriella Cusella''' is the leader of the wetlab in which the students | '''Gabriella Cusella''' is the leader of the wetlab in which the students | ||
worked during the summer. She organized the spaces, the instruments | worked during the summer. She organized the spaces, the instruments | ||
and the lab funds dedicated to the iGEM project. | and the lab funds dedicated to the iGEM project. | ||
+ | |||
'''Paolo Magni''' coordinated all the project activities: meetings, project | '''Paolo Magni''' coordinated all the project activities: meetings, project | ||
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problems and issues. | problems and issues. | ||
- | All team members contributed equally to brainstorming sessions, | + | |
- | BioBrick parts selection and project documentation. | + | '''All team members contributed equally to brainstorming sessions, BioBrick parts selection and project documentation.''' |
===Who did what=== | ===Who did what=== | ||
- | Integrative vector for E. coli: | + | ''Integrative vector for E. coli:'' |
- | + | ||
- | + | *Manuel L. and Manuel Z. had the idea. | |
+ | *Nicolò, and Matteo (supervised by Lorenzo) designed the vector and | ||
the experiments. | the experiments. | ||
- | + | *Nicolò and Federica (supervised by Lorenzo and Matteo) constructed | |
the clones and performed all the integration experiments. | the clones and performed all the integration experiments. | ||
- | Integrative vector for yeast: | + | |
- | + | ''Integrative vector for yeast:'' | |
+ | |||
+ | *Federica had the idea, designed the vector, cloned the vector in E. | ||
coli, designed and performed integration experiments in S. cerevisiae. | coli, designed and performed integration experiments in S. cerevisiae. | ||
- | + | *Susanna helped Federica during the genome analysis of S288C strain | |
to design the two integration sequences. | to design the two integration sequences. | ||
- | + | *Susanna and Manuel L. supervised the validation experiments. | |
- | Self-inducible devices: | + | |
- | + | ''Self-inducible devices:'' | |
- | + | ||
+ | *Lorenzo, Susanna and Nicolò had the idea. | ||
+ | *Nicolò (supervised by Susanna) designed and constructed all the | ||
devices. Nicolò also designed (supervised by Matteo and Susanna) and | devices. Nicolò also designed (supervised by Matteo and Susanna) and | ||
performed (supervised by Susanna) all the experiments. | performed (supervised by Susanna) all the experiments. | ||
- | + | *Nicolò analyzed the data. | |
- | PHB/Phasin binding: | + | ''PHB/Phasin binding:'' |
- | + | *Sara and Alessandro had the idea. | |
- | + | *Sara and Alessandro (supervised by Giacomo) designed the phasin | |
fusion proteins. | fusion proteins. | ||
- | + | *Giacomo and Manuel L. supervised the construction of the clones for | |
validation. The physical construction was performed by Sara, Federica | validation. The physical construction was performed by Sara, Federica | ||
and Manuel Z. | and Manuel Z. | ||
- | + | *Sara and Federica (supervised by Susanna and Manuel L.) designed and | |
performed the experiments on PHB. | performed the experiments on PHB. | ||
- | + | *Federica and Manuel Z. (supervised by Susanna and Giacomo) performed | |
the experiments on phasin fusion proteins. | the experiments on phasin fusion proteins. | ||
- | Intein: | + | |
- | + | ''Intein:'' | |
- | + | ||
- | + | *Alessandro had the idea. | |
+ | *Sara designed the intein sequence. | ||
+ | *Sara and Alessandro (supervised by Giacomo) designed the intein | ||
fusion proteins. | fusion proteins. | ||
- | + | *Giacomo supervised the construction of the clones for validation. | |
The physical construction was performed by Sara and Federica. | The physical construction was performed by Sara and Federica. | ||
- | + | *Nicolò (supervised by Giacomo) performed the experiments on intein | |
fusion proteins. | fusion proteins. | ||
Revision as of 08:59, 24 October 2010
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