Team:UNIPV-Pavia/Team/WwdPavia
From 2010.igem.org
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- | <table border="0 | + | <table border="0"><tr><td align="center" valign="top" colspan="3" style="padding:20px"> |
- | + | '''Riccardo Barini''' worked on: all the activities connected with wiki | |
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- | Riccardo Barini worked on: all the activities connected with wiki | + | |
design, updating and freezer management. He also helped the team | design, updating and freezer management. He also helped the team | ||
during the data analysis of quantitative experiments on self-inducible | during the data analysis of quantitative experiments on self-inducible | ||
devices. | devices. | ||
- | Sara Bisi worked on: the design of intein sequence, as well as the | + | |
+ | '''Sara Bisi''' worked on: the design of intein sequence, as well as the | ||
design and construction of protein fusions with phasin and intein. She | design and construction of protein fusions with phasin and intein. She | ||
- | also designed and performed (with Federica) validation experiments for | + | also designed and performed (with '''Federica''') validation experiments for |
PHB granules production. She participated to the fund raising | PHB granules production. She participated to the fund raising | ||
activities to gain financial aid from private companies. She had the | activities to gain financial aid from private companies. She had the | ||
- | idea (with Alessandro) of using PHB as a solid substrate for | + | idea (with '''Alessandro''') of using PHB as a solid substrate for |
phasin-tagged protein purification. | phasin-tagged protein purification. | ||
- | Nicolò Politi worked on: all the activities | + | |
+ | '''Nicolò Politi''' worked on: all the activities | ||
(design/construction/experimental validation and data analysis) | (design/construction/experimental validation and data analysis) | ||
connected with the self-inducible devices. He also had the idea | connected with the self-inducible devices. He also had the idea | ||
- | (with Susanna and Lorenzo) of the design of self-inducible promoters. | + | (with '''Susanna''' and '''Lorenzo''') of the design of self-inducible promoters. |
He helped during the design of the integrative vector for E. coli | He helped during the design of the integrative vector for E. coli | ||
- | and performed with Federica the validation experiments on it. | + | and performed with '''Federica''' the validation experiments on it. |
Finally, he performed fluorescence assays on intein fusion proteins. | Finally, he performed fluorescence assays on intein fusion proteins. | ||
- | Alessandro Ranieri worked on: the design of phasin and intein fusion | + | '''Alessandro Ranieri''' worked on: the design of phasin and intein fusion |
- | proteins. He had the idea (with Sara) of using PHB as a solid | + | proteins. He had the idea (with '''Sara''') of using PHB as a solid |
substrate for phasin-tagged protein purification. He also had the idea | substrate for phasin-tagged protein purification. He also had the idea | ||
of using an engineered inducible intein to cleave the tag after the | of using an engineered inducible intein to cleave the tag after the | ||
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shipping vector and performed the quality control on them. | shipping vector and performed the quality control on them. | ||
- | Federica Sampietro worked on: all the activities connected with | + | '''Federica Sampietro''' worked on: all the activities connected with |
invention/design/construction/experimental validation of the yeast | invention/design/construction/experimental validation of the yeast | ||
integrative vector. She worked on the construction of parts for the | integrative vector. She worked on the construction of parts for the | ||
integrative vector for E. coli, PHB/phasin and intein sub-projects. | integrative vector for E. coli, PHB/phasin and intein sub-projects. | ||
She also designed and performed the validation experiments on PHB | She also designed and performed the validation experiments on PHB | ||
- | granules production (with Sara) and the validation experiments on | + | granules production (with '''Sara''') and the validation experiments on |
- | phasin binding (with Manuel Z.). | + | phasin binding (with '''Manuel Z.'''). |
- | Chiara Tassorelli performed: an accurate analysis of past iGEM | + | '''Chiara Tassorelli''' performed: an accurate analysis of past iGEM |
projects during the first steps of our activities, as well as the | projects during the first steps of our activities, as well as the | ||
analysis of the literature for all the selected subprojects. She is | analysis of the literature for all the selected subprojects. She is | ||
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designed the logo and worked on wiki design. | designed the logo and worked on wiki design. | ||
- | Manuel Zocco worked on: the construction of phasin fusion proteins | + | '''Manuel Zocco''' worked on: the construction of phasin fusion proteins |
- | (with Sara and Federica) and performed the experiments on PHB/phasin | + | (with Sara and '''Federica''') and performed the experiments on PHB/phasin |
- | binding (with Federica). He also helped during validation experiments | + | binding (with '''Federica'''). He also helped during validation experiments |
by PCR on the integrative vector for E. coli and he had the idea (with | by PCR on the integrative vector for E. coli and he had the idea (with | ||
- | Manuel L.) of the integrative vector for E. coli. | + | '''Manuel L.''') of the integrative vector for E. coli. |
- | Manuel Lupotto had the idea of the integrative vector for E. coli (with | + | '''Manuel Lupotto''' had the idea of the integrative vector for E. coli (with |
- | Manuel Z.). He supervised part of the every day work in the wetlab during | + | '''Manuel Z.'''). He supervised part of the every day work in the wetlab during |
cloning and experimental validation activities. He also supervised the | cloning and experimental validation activities. He also supervised the | ||
experiments about PHB granules production validation and the yeast integrative | experiments about PHB granules production validation and the yeast integrative | ||
- | vector validation (with Susanna). Primer design supervisor. | + | vector validation (with '''Susanna'''). Primer design supervisor. |
- | Matteo Meroso supervised all the activities | + | '''Matteo Meroso''' supervised all the activities |
(design/construction/experimental validation) connected with the | (design/construction/experimental validation) connected with the | ||
- | integrative vector for E. coli. He also supervised (with Susanna) the | + | integrative vector for E. coli. He also supervised (with '''Susanna''') the |
design of experiments for the self-inducible devices. | design of experiments for the self-inducible devices. | ||
- | Lorenzo Pasotti coordinated the team during the brainstorming | + | '''Lorenzo Pasotti''' coordinated the team during the brainstorming |
sessions, project discussions and during the submission and | sessions, project discussions and during the submission and | ||
documentation of BioBrick parts to the Registry. Instructor for the | documentation of BioBrick parts to the Registry. Instructor for the | ||
rest of the team about physical and functional standardization in | rest of the team about physical and functional standardization in | ||
- | Synthetic Biology. He had the idea (with Susanna and Nicolò) of the design of | + | Synthetic Biology. He had the idea (with '''Susanna''' and '''Nicolò''') of the design of |
self-inducible devices. | self-inducible devices. | ||
- | Giacomo Zambianchi supervised the design and construction of phasin | + | '''Giacomo Zambianchi''' supervised the design and construction of phasin |
and intein fusion proteins, as well as the test constructs and the | and intein fusion proteins, as well as the test constructs and the | ||
validation experiments. Instructor for the rest of the team about wiki | validation experiments. Instructor for the rest of the team about wiki | ||
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coordinator and the advisor of the fundraising activities. | coordinator and the advisor of the fundraising activities. | ||
- | Susanna Zucca planned and coordinated all the student and advisor | + | '''Susanna Zucca''' planned and coordinated all the student and advisor |
activities in both wetlab and drylab and trained the students for the | activities in both wetlab and drylab and trained the students for the | ||
cloning and quantitative experiments. She also had the idea (with | cloning and quantitative experiments. She also had the idea (with | ||
- | Lorenzo and Nicolò) of the design of self-inducible devices. She supervised the | + | '''Lorenzo''' and '''Nicolò''') of the design of self-inducible devices. She supervised the |
cloning and experimental validation activities in integrative vector | cloning and experimental validation activities in integrative vector | ||
for yeast, self-inducible devices and part of the PHB/phasin and | for yeast, self-inducible devices and part of the PHB/phasin and | ||
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promoter measurement, modeling and data analysis. | promoter measurement, modeling and data analysis. | ||
- | Gabriella Cusella is the leader of the wetlab in which the students | + | '''Gabriella Cusella''' is the leader of the wetlab in which the students |
worked during the summer. She organized the spaces, the instruments | worked during the summer. She organized the spaces, the instruments | ||
and the lab funds dedicated to the iGEM project. | and the lab funds dedicated to the iGEM project. | ||
- | Paolo Magni coordinated all the project activities: meetings, project | + | '''Paolo Magni''' coordinated all the project activities: meetings, project |
timeline, fundraising, contacts with media and institutions. Registry, | timeline, fundraising, contacts with media and institutions. Registry, | ||
wiki, poster and presentation supervisor (with the other advisors). He | wiki, poster and presentation supervisor (with the other advisors). He | ||
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- | Who did what | + | ===Who did what=== |
Integrative vector for E. coli: | Integrative vector for E. coli: | ||
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- Nicolò (supervised by Giacomo) performed the experiments on intein | - Nicolò (supervised by Giacomo) performed the experiments on intein | ||
fusion proteins. | fusion proteins. | ||
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+ | </td></tr> | ||
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+ | </table> | ||
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+ | </td></tr></table> |
Revision as of 08:56, 24 October 2010
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