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| | = Week 2 13th September - 17th 2010 Holidays in México = | | = Week 2 13th September - 17th 2010 Holidays in México = |
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| - | = Week 3 18th September -22th 2010 =
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| - | ==''Monday''==
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| - | ==='''Planned and prepared for tomorrow's electroporation'''===
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| - | *'''Depart electroporator.'''
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| - | *'''Made up LB agar plates and cloramphenicol 100μl.'''
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| - | *'''Plan to transform Psb1C3 froma plate.'''
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| - | ==='''We were looking for a nanodrop to quantify DNA’s concentrations'''===
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| - | ==='''and tried to make ligations Psb1C3 with each one of primers'''===
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| - | ==='''(our this year's bricks).'''===
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| - | ==''Tuesday''==
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| - | ==='''Transformed Top 10 competent cells, and did 10 plates incubated overnight.'''===
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| - | ==''Wednsday''==
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| - | ==='''Results previous day transformations only two plates were completed unsucessful.'''===
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| - | *'''Prepared solutions for miniprep via Alcalin lisis method'''
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| - | [[Image:Imagen2.gif]]
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| - | ==''Thursday''==
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| - | ==='''The inoculum for miniprep we prepared 6 vector’s vials'''===
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| - | ==='''without positive results. Maybe the solutions for alcaline'''===
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| - | ==='''lisis are not well.'''===
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| - | [[Image:Imagen3.gif|500px]]
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| - | *'''We have discused over how to quantify our cold sistem response'''
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| - | '''we gonig to use a promoter sistem, the funcional casset using J13002 as follow'''
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| - | *'''Promoter + our functional secuence + GFP'''
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| - | [[Image:Cuant1.gif]]
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| - | *'''Promoter + our functional secuence + AFP (Anty freeze protein)
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| - | [[Image:Imagen4.gif]]
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| - | *'''We have a meeting with our Genomics frend’s we going to get a
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| - | '''colaboration on Modeling and probably at wet lab too.
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