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Team:Harvard/fences/notebook
From 2010.igem.org
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==06-14-2010 [ [[#top|top]] ]== | ==06-14-2010 [ [[#top|top]] ]== | ||
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| - | + | <b>LacI Transformation</b> | |
| - | + | Performed bacterial transformation according to [[Silver:_Bacterial_Transformation]], however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL. | |
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| + | Transformed the following, each into its own tube of TOP10 E.Coli: | ||
| + | 1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2 | ||
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| + | 2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3 | ||
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| + | Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells. | ||
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| + | Plates incubated overnight, colonies observed in all plates except the control. | ||
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| + | [[Image:Colonies 6-15-10.jpg|300px]] | ||
| + | [[Image:Colonies-2 6-15-10.jpg|300px]] | ||
| + | [[Image:Colonies-3 6-15-10.jpg|300px]] | ||
==06-15-2010 [ [[#top|top]] ]== | ==06-15-2010 [ [[#top|top]] ]== | ||
Revision as of 18:52, 23 October 2010
notebook calendar
06-14-2010 [ top ]
LacI Transformation
Performed bacterial transformation according to Silver:_Bacterial_Transformation, however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL.
Transformed the following, each into its own tube of TOP10 E.Coli:
1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2
2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3
Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells.
Plates incubated overnight, colonies observed in all plates except the control.
