Team:Stockholm/23 October 2010

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(New page: {{Stockholm/Top2}} ==Andreas== ===IgG protease activity assay===)
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===IgG protease activity assay===
===IgG protease activity assay===
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Finished the repeated experiment and read the wavelength at 450 nm.
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'''Blank:''' 80 μl PBS + 100 μl SureBlue™ peroxidase substrate + 100 μl 1 M HCl.
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{|border="1" cellpadding="1" cellspacing="0"
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!colspan="14"|Sample loading
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|-
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!width="50"|Ab dil.
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|width="50"| 
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!width="50"|1
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!width="50"|2
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!width="50"|3
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!width="50"|4
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!width="50"|5
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!width="50"|6
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!width="50"|11
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!width="50"|12
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|-
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!1:500
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!A
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|colspan="2" align="center"|Ind. IgGp A
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|colspan="2" align="center"|Ind. IgGp B
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|colspan="2" align="center"|Ind. SOD A
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|colspan="2" align="center"|Ind. SOD B
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|colspan="2" align="center"|Unind. IgGp A
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|colspan="2" align="center"|Unind. IgGp B
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|-
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!1:250
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!B
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|colspan="2" align="center"|Ind. IgGp A
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|colspan="2" align="center"|Ind. IgGp B
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|colspan="2" align="center"|Ind. SOD A
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|colspan="2" align="center"|Ind. SOD B
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|colspan="2" align="center"|Unind. IgGp A
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|colspan="2" align="center"|Unind. IgGp B
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|-
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!–
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!C
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|align="center"|PBS 1:500
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|align="center"|PBS 1:250
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|align="center"|Blank
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|}
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====Results====
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{|border="1" cellpadding="1" cellspacing="0"
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!colspan="13"|A<sub>450</sub> measurements
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|-
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|width="50"|&nbsp;
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!width="50"|1
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!width="50"|2
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!width="50"|3
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!width="50"|10
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!width="50"|11
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!width="50"|12
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|-
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!A
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|align="center"|0.073
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|align="center"|0.075
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|align="center"|0.087
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|align="center"|0.087
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|align="center"|0.091
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|align="center"|0.094
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|align="center"|0.089
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|align="center"|0.090
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|align="center"|0.131
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|align="center"|0.132
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|align="center"|0.109
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|align="center"|0.108
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|-
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!B
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|align="center"|0.116
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|align="center"|0.126
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|align="center"|0.116
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|align="center"|0.117
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|align="center"|0.156
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|align="center"|0.158
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|align="center"|0.145
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|align="center"|0.150
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|align="center"|0.181
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|align="center"|0.187
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|align="center"|0.125
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|align="center"|0.124
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|-
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!C
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|align="center"|0.003
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|align="center"|0.011
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|align="center"|'''0.000'''
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|}

Revision as of 15:21, 23 October 2010

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Andreas

IgG protease activity assay

Finished the repeated experiment and read the wavelength at 450 nm.

Blank: 80 μl PBS + 100 μl SureBlue™ peroxidase substrate + 100 μl 1 M HCl.

Sample loading
Ab dil.   1 2 3 4 5 6 7 8 9 10 11 12
1:500 A Ind. IgGp A Ind. IgGp B Ind. SOD A Ind. SOD B Unind. IgGp A Unind. IgGp B
1:250 B Ind. IgGp A Ind. IgGp B Ind. SOD A Ind. SOD B Unind. IgGp A Unind. IgGp B
– C PBS 1:500 PBS 1:250 Blank – – – – – – – – –

Results

A450 measurements
  1 2 3 4 5 6 7 8 9 10 11 12
A 0.073 0.075 0.087 0.087 0.091 0.094 0.089 0.090 0.131 0.132 0.109 0.108
B 0.116 0.126 0.116 0.117 0.156 0.158 0.145 0.150 0.181 0.187 0.125 0.124
C 0.003 0.011 0.000 – – – – – – – – –
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