Team:Groningen/5 July 2010

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Team:Groningen/Header}}
{{Team:Groningen/Header}}
-
'''Week 27'''
+
'''Week 27, Arend Jan'''
-
The pMASK-EH construct is cut with EcoRI and SpeI to get the fragments containing the ChpE and ChpH genes and ligate them in to the expression plasmid. A mix of both genes will be used for ligation and the clones will be checked for which gene they contain.  
+
 
 +
The pMASK-EH construct is cut with EcoRI and SpeI to get the fragments containing the ChpE and ChpH genes and ligate them in to the expression plasmid. A mix of both genes will be used for ligation and the clones will be checked afterwards for which gene they contain.  

Revision as of 15:08, 23 October 2010

iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

Week 27, Arend Jan


The pMASK-EH construct is cut with EcoRI and SpeI to get the fragments containing the ChpE and ChpH genes and ligate them in to the expression plasmid. A mix of both genes will be used for ligation and the clones will be checked afterwards for which gene they contain.


- 10ul plasmid pNZ8901-bbs
- 1.5ul buffer Tango
- 0.5ul SpeI
- 3ul MQ
- perform digestion
- add 1.88ul buffer Tango
- add 0.5ul EcoRI

- 17.5ul plasmid pMASK-EH
- 2ul buffer Tango
- 0.5ul SpeI
- perform digestion
- add 2.5ul buffer Tango
- add 0.5ul EcoRI


Mix of fragments containing ChpE and ChpH genes at ~300bp
Restriction of expression backbone with EcoRI and SpeI



Tried to cut out the insertion from pNZ8901 and replace it with a part from a Plasmid with an identical backbone, all attempts failed however.




Search

 

iGEM 2010 main page
iGEM HQ
iGEM Groningen Team page

University of Groningen
Where on earth are we?
Share |