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Team:Groningen/5 July 2010
From 2010.igem.org
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'''Week 27''' | '''Week 27''' | ||
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| + | The pMASK-EH construct is cut with EcoRI and SpeI to get the fragments containing the ChpE and ChpH genes and ligate them in to the expression plasmid. A mix of both genes will be used for ligation and the clones will be checked for which gene they contain. | ||
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| + | [[Image:08-07-10gn.jpg|200px|thumb|left|Mix of fragments containing ChpE and ChpH genes]] [[Image:08-07-10-2gn.jpg|200px|thumb|left|alt text]] | ||
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Tried to cut out the insertion from pNZ8901 and replace it with a part from a Plasmid with an identical backbone, all attempts failed however. | Tried to cut out the insertion from pNZ8901 and replace it with a part from a Plasmid with an identical backbone, all attempts failed however. | ||
Revision as of 15:01, 23 October 2010
Hydrophobofilm
pushing coatings into a greener future
Week 27
The pMASK-EH construct is cut with EcoRI and SpeI to get the fragments containing the ChpE and ChpH genes and ligate them in to the expression plasmid. A mix of both genes will be used for ligation and the clones will be checked for which gene they contain.
Tried to cut out the insertion from pNZ8901 and replace it with a part from a Plasmid with an identical backbone, all attempts failed however.
