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BIOTEC Dresden/Notepad/12 October 2010
From 2010.igem.org
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| + | '''Fusion Protein''' | ||
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| + | Colony PCR for the transformed pETMM43. | ||
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| + | Gel purification of plasmids pSB1T3 and pSB1C3 | ||
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| + | Miniprep of parts luxI + zz in pETMMzz as well as miniprep of pETMM43 plasmid | ||
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| + | Ligations of parts 34 and 35 for the pigment assembly and their transformation | ||
<hr> | <hr> | ||
Revision as of 12:09, 23 October 2010
Parts Assembly
Minipreps of the overnight cultures were done and the same were given for sequencing in order to confirm the presence of the desired insert.
52a, 52b, 52c
The following parts were taken from glycerol stock and kept for overnight incubation at 37 degrees.
1a, 4a
Fusion Protein
Colony PCR for the transformed pETMM43.
Gel purification of plasmids pSB1T3 and pSB1C3
Miniprep of parts luxI + zz in pETMMzz as well as miniprep of pETMM43 plasmid
Ligations of parts 34 and 35 for the pigment assembly and their transformation
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
