Team:Osaka/Protocols

From 2010.igem.org

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<h2>Protocols</h2>
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==Protocols==
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<li>Preparation of competent cells for heat-shock transformation
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<li>Transformation by heat-shock
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*Preparation of competent cells for heat-shock transformation
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<li>Plasmid DNA miniprep
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*Transformation by heat-shock
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<li>3A BioBrick assembly
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*Plasmid DNA miniprep
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<li>PCR cloning
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*3A BioBrick assembly
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<li>PCR site-directed mutagenesis
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*PCR cloning
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<li>Extraction of genome DNA for PCR cloning
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*PCR site-directed mutagenesis
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*Extraction of genome DNA for PCR cloning
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Revision as of 10:45, 23 October 2010


Protocols

  • Preparation of competent cells for heat-shock transformation
  • Transformation by heat-shock
  • Plasmid DNA miniprep
  • 3A BioBrick assembly
  • PCR cloning
  • PCR site-directed mutagenesis
  • Extraction of genome DNA for PCR cloning