Team:Osaka/Protocols
From 2010.igem.org
(Difference between revisions)
Kagatataku (Talk | contribs) |
|||
Line 2: | Line 2: | ||
<html> | <html> | ||
<div class="contents2"> | <div class="contents2"> | ||
- | + | </html> | |
- | + | ==Protocols== | |
- | + | ||
- | + | *Preparation of competent cells for heat-shock transformation | |
- | + | *Transformation by heat-shock | |
- | + | *Plasmid DNA miniprep | |
- | + | *3A BioBrick assembly | |
- | + | *PCR cloning | |
- | + | *PCR site-directed mutagenesis | |
- | < | + | *Extraction of genome DNA for PCR cloning |
+ | <html> | ||
</div> | </div> | ||
</html> | </html> |
Revision as of 10:45, 23 October 2010
Protocols
- Preparation of competent cells for heat-shock transformation
- Transformation by heat-shock
- Plasmid DNA miniprep
- 3A BioBrick assembly
- PCR cloning
- PCR site-directed mutagenesis
- Extraction of genome DNA for PCR cloning