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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10settembre bis
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control) | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control) | ||
* <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | * <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | ||
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| - | ==RESULTS | + | Supernatants were properly diluted in order to evaluate the production of 3OC6-HSL of the low copy number parts. |
| + | |||
| + | ==RESULTS== | ||
| + | |||
| + | The 3OC6-HSL concentrations of several cultures were evaluated: | ||
| + | |||
| + | |||
| + | [[Image:UNIPV_Pavia_Serie110.png| 620px | center ]] | ||
| + | |||
| + | |||
| + | [[Image:UNIPV_Pavia_Serie210.png| 620px | center ]] | ||
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| + | |||
| + | [[Image:UNIPV_Pavia_Serie310.png| 620px | center ]] | ||
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| + | Concentrations of 3OC6-HSL were obtained multiplying the values read on the graphics for the corresponding dilution factor. | ||
| + | |||
| + | {|border='1' align='center' | ||
| + | || Part || 3OC6-HSL concentration [uM] | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.4 | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.02 | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.1 | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> || ~0.002 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.005 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.002 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.003 | ||
| + | |} | ||
| + | |||
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Latest revision as of 10:27, 23 October 2010
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