Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre

From 2010.igem.org

(Difference between revisions)
(RESULTS (?))
(RESULTS)
 
(2 intermediate revisions not shown)
Line 40: Line 40:
* <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)
* <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)
-
The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]].
+
The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. All supernatants were diluted 1:100.
==RESULTS==
==RESULTS==
Line 51: Line 51:
|| Part || 3OC6-HSL concentration [uM]
|| Part || 3OC6-HSL concentration [uM]
|-
|-
-
|| ||  
+
|| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.7
|-
|-
-
|| ||  
+
|| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.04
|-
|-
-
|| ||  
+
|| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.09
|}
|}
-
Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the next test.
+
Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the [[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test10settembre_bis|next test]].
<!-- table previous next test -->
<!-- table previous next test -->

Latest revision as of 09:55, 23 October 2010

SEPTEMBER, 8TH



PLATE


UNIPV Pavia piastra8settembre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to measure the 3OC6-HSL concentration due to the synthesis of homoserine-lactone by 3OC6-HSL generators in saturation phase of the cultures growth.

Methods

According to Protocol 3 the experiment was performed in LB on the following signalling parts:

  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19-4C5)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control)
  • <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)

The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in Protocol 3. All supernatants were diluted 1:100.

RESULTS

UNIPV Pavia Serie18.png

It was possible to evaluate some of the 3OC6-HSL concentrations:

Part 3OC6-HSL concentration [uM]
<partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> ~0.7
<partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> ~0.04
<partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> ~0.09

Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the next test.



Tecan Test