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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre
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<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | ||
| - | <html><p align="center"><font size="5"><b> | + | <html><p align="center"><font size="5"><b>SEPTEMBER, 8TH</b></font></p></html><hr><br> |
==PLATE== | ==PLATE== | ||
<br> | <br> | ||
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control) | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control) | ||
* <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | * <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | ||
| - | |||
| - | The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. | + | The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. All supernatants were diluted 1:100. |
| - | ==RESULTS | + | ==RESULTS== |
| + | |||
| + | [[Image:UNIPV_Pavia_Serie18.png| 620px | center ]] | ||
| + | |||
| + | It was possible to evaluate some of the 3OC6-HSL concentrations: | ||
| + | |||
| + | {| border='1' align='center' | ||
| + | || Part || 3OC6-HSL concentration [uM] | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.7 | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.04 | ||
| + | |- | ||
| + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.09 | ||
| + | |} | ||
| + | |||
| + | Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the [[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test10settembre_bis|next test]]. | ||
<!-- table previous next test --> | <!-- table previous next test --> | ||
Latest revision as of 09:55, 23 October 2010
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