Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre

From 2010.igem.org

(Difference between revisions)
(→Methods)
(→RESULTS)
 
(6 intermediate revisions not shown)
Line 11: Line 11:
<td valign="top">
<td valign="top">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
-
<html><p align="center"><font size="5"><b>19° TEST</b></font><br><br><font size="4">[SEPTEMBER, 8TH]</font></p></html><hr><br>
+
<html><p align="center"><font size="5"><b>SEPTEMBER, 8TH</b></font></p></html><hr><br>
==PLATE==
==PLATE==
<br>
<br>
Line 39: Line 39:
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control)
* <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)
* <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)
-
* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_K</partinfo> (negative control)
 
-
The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]].
+
The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. All supernatants were diluted 1:100.
-
==RESULTS (?)==
+
==RESULTS==
 +
 
 +
[[Image:UNIPV_Pavia_Serie18.png| 620px | center ]]
 +
 
 +
It was possible to evaluate some of the 3OC6-HSL concentrations:
 +
 
 +
{| border='1' align='center'
 +
|| Part || 3OC6-HSL concentration [uM]
 +
|-
 +
|| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.7
 +
|-
 +
|| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.04
 +
|-
 +
|| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.09
 +
|}
 +
 
 +
Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the [[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test10settembre_bis|next test]].
<!-- table previous next test -->
<!-- table previous next test -->
Line 60: Line 75:
<td align="left" valign="top" width="15%">
<td align="left" valign="top" width="15%">
-
<table border="0" width="100%" height="100%">
+
{{UNIPV-Pavia/Menu_measurments}}
-
<tr align="right"><td height="15">
+
-
[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
+
-
</td></tr>
+
-
<tr align="right"><td height="15">
+
-
[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
+
-
</td></tr>
+
-
</table>
+
-
 
+
</td>
</td>
</table>
</table>

Latest revision as of 09:55, 23 October 2010
Home
The Project
Materials & Methods
Parts
The Team
Notebook
Biological Safety
Pavia
Gallery
Sponsors

SEPTEMBER, 8TH


PLATE


UNIPV Pavia piastra8settembre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to measure the 3OC6-HSL concentration due to the synthesis of homoserine-lactone by 3OC6-HSL generators in saturation phase of the cultures growth.

Methods

According to Protocol 3 the experiment was performed in LB on the following signalling parts:

  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18)
  • <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19-4C5)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control)
  • <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)

The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in Protocol 3. All supernatants were diluted 1:100.

RESULTS

UNIPV Pavia Serie18.png

It was possible to evaluate some of the 3OC6-HSL concentrations:

Part 3OC6-HSL concentration [uM]
<partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> ~0.7
<partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> ~0.04
<partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> ~0.09

Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the next test.



Previous Test Next Test

Tecan Test

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test8settembre"