Team:Northwestern/Protocol

From 2010.igem.org

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(DNA)
(Bacterial Work)
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===[[Transformation]]===

Revision as of 23:10, 24 June 2010

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Home Team Project Parts Notebook Safety Calendar Protocol

Protocol

http://ginkgobioworks.com/support/

Formatting: http://meta.wikimedia.org/wiki/Help:Wikitext_examples

OpenWetWare Protocols: http://openwetware.org/wiki/Protocols


Contents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep