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User:HassanForoughiAsl/22 June 2010
From 2010.igem.org
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| - | + | ;EuMelanin (EM): | |
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#Melanin Gene in recombinant E. Coli. | #Melanin Gene in recombinant E. Coli. | ||
#How IPTG effecting EM production. | #How IPTG effecting EM production. | ||
| - | #Study this article: | + | #Study this article: http://www.ncbi.nlm.nih.gov/pubmed/17040223 Lagunas-Muñoz VH et. al. 2006 : |
| - | :*;Ask wetlab team for what their PH, Temp, and IPTG is for their bacteria. | + | #:*Suggests that temperature and PH are the main factors effecting melanin production. they reached a 100% increase in melanin production by changing PH and Temp. |
| + | #:*The production of melanin is subjected to the presence of tyrosinase, which is an enzyme to catalyze the oxidation of phenols (for the melanin case this one is tyrosine) source: wikipedia. | ||
| + | #:*These enzymes have two activities: they use molecular oxygen to catalyse the hydroxylation of l-tyrosine to l-3,4- dihydroxyphenyl alanine (l-dopa) (cresolase activity); and then oxidizes this product to dopachrome (catecholase activity), which then non-enzymatically oxidizes and polymerizes to form melanin (Garcı´a-Borro´n and Solano 2002). | ||
| + | #:*EuMel production is what they were interested in, EuMel is directly related to induction of MutMelA gene, which could be controlled by concentration of IPTG. They suggest a 0.1 mmol/litr IPTG for mel production. | ||
| + | #;Ask wetlab team for what their PH, Temp, and IPTG is for their bacteria. | ||
#Possible pathway in EM production in E. Coli. | #Possible pathway in EM production in E. Coli. | ||
| + | #What else is needed to represent the whole as system!? | ||
| + | |||
| + | |||
| + | ;Overexpression / inclusion bodies | ||
| + | |||
| + | #TODO: | ||
| + | #:Read about inclusion bodies, over expression. | ||
| + | #:Read articles in production part of modeling and add more/delete some. | ||
Latest revision as of 20:48, 24 June 2010
- EuMelanin (EM)
- Melanin Gene in recombinant E. Coli.
- How IPTG effecting EM production.
- Study this article: http://www.ncbi.nlm.nih.gov/pubmed/17040223 Lagunas-Muñoz VH et. al. 2006 :
- Suggests that temperature and PH are the main factors effecting melanin production. they reached a 100% increase in melanin production by changing PH and Temp.
- The production of melanin is subjected to the presence of tyrosinase, which is an enzyme to catalyze the oxidation of phenols (for the melanin case this one is tyrosine) source: wikipedia.
- These enzymes have two activities: they use molecular oxygen to catalyse the hydroxylation of l-tyrosine to l-3,4- dihydroxyphenyl alanine (l-dopa) (cresolase activity); and then oxidizes this product to dopachrome (catecholase activity), which then non-enzymatically oxidizes and polymerizes to form melanin (Garcı´a-Borro´n and Solano 2002).
- EuMel production is what they were interested in, EuMel is directly related to induction of MutMelA gene, which could be controlled by concentration of IPTG. They suggest a 0.1 mmol/litr IPTG for mel production.
- Ask wetlab team for what their PH, Temp, and IPTG is for their bacteria.
- Possible pathway in EM production in E. Coli.
- What else is needed to represent the whole as system!?
- Overexpression / inclusion bodies
- TODO:
- Read about inclusion bodies, over expression.
- Read articles in production part of modeling and add more/delete some.
