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Team:Harvard/vectors
From 2010.igem.org
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<h1>abstract</h1> | <h1>abstract</h1> | ||
| - | <p>Our | + | <p>Our aim in creating the iGarden was to create a framework for plant engineering using the BioBrick standard. To do so, we created a set of vectors fitting the BioBrick standard into which we place out BioBrick constructs with which we can transfer the constructs from E. coli, our assembly chassis, to the plant, our final chassis. We make this transfer via agrobacterium-mediated transformation.</p> |
<p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p> | <p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p> | ||
Revision as of 01:37, 23 October 2010
abstract lab notebook agrobacteria and plant engineering meet the vectors plant transformation parts & primers references
abstract
Our aim in creating the iGarden was to create a framework for plant engineering using the BioBrick standard. To do so, we created a set of vectors fitting the BioBrick standard into which we place out BioBrick constructs with which we can transfer the constructs from E. coli, our assembly chassis, to the plant, our final chassis. We make this transfer via agrobacterium-mediated transformation.
The process began with the creation of desired constructs in e. coli - such as flavor elements, allergen knockdown, pigment accumulation, or fence mechanisms. We next built a set of plant vectors compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.
