Team:Northwestern/Protocol

@@ Line 188: / Line 188: @@
 [[Team:Northwestern/Protocol/LIVE/DEAD® BacLight - Bacterial Viability Kit|Live/Dead Baclight]]
-===[[Prepping DNA from the kit plates]]===
-===[[Quikchange (from primers to colonies!)]]===
-===[[Kit to Stock Plasmid]]===
-===[[Ethanol Precipitation]]===
-===[[New Part Design(PCR)]]===
-=='''Bacterial Work'''==
-===[[Transformation]]===
-===[[O/N Culture]]===
-===[[Preparation of Competent Cells]]===
-===[[LB Media]]===
-===[[Preparing Plates]]===
-===[[Glycerol Stocks]]===
-=='''Assembly'''==
-===[[Restriction Enzyme Digests]]===
-===[[Plasmid Construction]]===
-===[[3A Assembly]]===
-===[[Ligations]]===
-===[[Mini Prep]]===
-=='''Microscopy'''==
-===[[Confocal Microscopy]]===
-===[[Fluorescence Microscopy]]===
-=='''Reagents'''==
-===[[Reagents]]===
-=='''Cell Staining'''==
-===[[Rhodamine-Conjugated Chitin Probe]]===
-==Methanol Fixation==
-# Rinse slide with ethanol and flame
-# Rinse slide with PBS and dry with KimWipe
-# Apply cell sample to microscope slide and let air dry
-# Submerge in -20C absolute methanol for 5-10 min
-# Wash 3 times with 1X TBS (submerge in 1X TBS for 5 minutes per wash)
-** '''Do not let cells dry for the rest of the procedure'''
-===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===
 |}