Team:Northwestern/References

From 2010.igem.org

(Difference between revisions)
Line 131: Line 131:
|
|
-
== Lac Operon Control ==
+
=== Lac Operon Control ===
Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG
Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG
Line 139: Line 139:
-
==Chitin==
+
===Chitin===
Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886
Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886
-
Chitin synthesis Pathway (image): http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg
+
Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg]
Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long  
Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long  
Line 168: Line 168:
Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html
Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html
-
== Apoptosis ==
+
=== Apoptosis ===
partsregistry: http://partsregistry.org/Cell_death
partsregistry: http://partsregistry.org/Cell_death
Line 179: Line 179:
*http://www.biotium.com/product/product_info/Protocol/30027.pdf
*http://www.biotium.com/product/product_info/Protocol/30027.pdf
-
==Keio Collection - KnockOuts==
+
===Keio Collection - KnockOuts===
Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html  
Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html  
Line 207: Line 207:
more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf
more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf
-
== BIF (Biological Imaging Facility)==
+
=== BIF (Biological Imaging Facility)===
List of Equipment: http://www.northwestern.edu/bioimaging/equip.html <br />
List of Equipment: http://www.northwestern.edu/bioimaging/equip.html <br />
Methods: http://www.northwestern.edu/bioimaging/methods.html
Methods: http://www.northwestern.edu/bioimaging/methods.html

Revision as of 01:19, 23 October 2010


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Lac Operon Control

Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG



Chitin

Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886

Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg]

Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long

Uses for Chitin: http://www.gmp-chitosan.com/en/products-services/chitin.html

Chitin Synthesis

Chitin Synthase Gene (CHS3): http://www.ncbi.nlm.nih.gov/gene/852311

CHS3 polymerizes: http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465


Chitin Staining (Calcofluor)

Effect on e. coli: http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf

Staining of Biofilm Polysaccharides- http://tinyurl.com/36pn4za

Inhibition of Chitin Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/

Data sheet: http://tinyurl.com/283wax2

Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html

Apoptosis

partsregistry: http://partsregistry.org/Cell_death


Live/Dead Staining aka Viability/Cytotoxicity Assay

DMAO(Live), EtD-III(Dead)

  • http://www.promokine.info/products/cell-analysis/cell-staining-reagents-related-products/
  • http://www.biotium.com/product/product_info/Protocol/30027.pdf

Keio Collection - KnockOuts

Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html

Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp

Method: http://www.pnas.org/content/97/12/6640.full.pdf+html

Journal Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html

Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls


ChiA (Chitinase) Knockout

Summary of e. coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

Chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

ydgg/tqsA (AI2-Transporter) Knockout

Knockout biofilm formation: http://jb.asm.org/cgi/content/short/188/2/587, http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593

more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf

BIF (Biological Imaging Facility)

List of Equipment: http://www.northwestern.edu/bioimaging/equip.html
Methods: http://www.northwestern.edu/bioimaging/methods.html