Team:Northwestern/References
From 2010.igem.org
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- | == Lac Operon Control == | + | === Lac Operon Control === |
Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG | Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG | ||
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- | ==Chitin== | + | ===Chitin=== |
Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886 | Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886 | ||
- | Chitin synthesis Pathway (image): http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg | + | Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg] |
Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long | Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long | ||
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Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html | Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html | ||
- | == Apoptosis == | + | === Apoptosis === |
partsregistry: http://partsregistry.org/Cell_death | partsregistry: http://partsregistry.org/Cell_death | ||
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*http://www.biotium.com/product/product_info/Protocol/30027.pdf | *http://www.biotium.com/product/product_info/Protocol/30027.pdf | ||
- | ==Keio Collection - KnockOuts== | + | ===Keio Collection - KnockOuts=== |
Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html | Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html | ||
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more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf | more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf | ||
- | == BIF (Biological Imaging Facility)== | + | === BIF (Biological Imaging Facility)=== |
List of Equipment: http://www.northwestern.edu/bioimaging/equip.html <br /> | List of Equipment: http://www.northwestern.edu/bioimaging/equip.html <br /> | ||
Methods: http://www.northwestern.edu/bioimaging/methods.html | Methods: http://www.northwestern.edu/bioimaging/methods.html |
Revision as of 01:19, 23 October 2010
Home | Brainstorm | Team | Acknowledgements | Project | Human Practices | Parts | Notebook | Calendar | Protocol | Safety | Links | References | Media | Contact |
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Lac Operon ControlLac Operon induction and IPTG: http://openwetware.org/wiki/IPTG
ChitinAdding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886 Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg] Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long Uses for Chitin: http://www.gmp-chitosan.com/en/products-services/chitin.html Chitin SynthesisChitin Synthase Gene (CHS3): http://www.ncbi.nlm.nih.gov/gene/852311 CHS3 polymerizes: http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465
Chitin Staining (Calcofluor)Effect on e. coli: http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf Staining of Biofilm Polysaccharides- http://tinyurl.com/36pn4za Inhibition of Chitin Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/ Data sheet: http://tinyurl.com/283wax2 Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html Apoptosispartsregistry: http://partsregistry.org/Cell_death
Live/Dead Staining aka Viability/Cytotoxicity AssayDMAO(Live), EtD-III(Dead)
Keio Collection - KnockOutsKeio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp Method: http://www.pnas.org/content/97/12/6640.full.pdf+html Journal Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls
ChiA (Chitinase) KnockoutSummary of e. coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts Chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837 chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 ydgg/tqsA (AI2-Transporter) KnockoutKnockout biofilm formation: http://jb.asm.org/cgi/content/short/188/2/587, http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593 more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf BIF (Biological Imaging Facility)List of Equipment: http://www.northwestern.edu/bioimaging/equip.html
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