"
Team:Newcastle/23 June 2010
From 2010.igem.org
(Difference between revisions)
(→Wednesday afternoon taking a break) |
RachelBoyd (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| + | ===Wednesday Morning=== | ||
| + | |||
| + | Cells are made competent ready for transformation. | ||
| + | *Positive control(plasmid uncut)- to make sure cells are transfroming properly | ||
| + | *Negative control (just cells)- nothing but competent cells | ||
| + | |||
| + | 5 microfuge tubes: | ||
| + | *1:5 | ||
| + | *1:3 | ||
| + | *Vector | ||
| + | *Negative add nothing | ||
| + | *Positive add 1μl uncut | ||
| + | |||
=== Wednesday afternoon taking a break === | === Wednesday afternoon taking a break === | ||
| Line 4: | Line 17: | ||
|- | |- | ||
|[[Image:Newcastle_Hoppings_001.JPG|thumb]] | |[[Image:Newcastle_Hoppings_001.JPG|thumb]] | ||
| - | |[[Image:Newcastle_Hoppings_002.JPG|thumb]] | + | |[[Image:Newcastle_Hoppings_002.JPG|thumb|Call to arms!]] |
|[[Image:Newcastle_Hoppings_003.JPG|thumb|Philamentous]] | |[[Image:Newcastle_Hoppings_003.JPG|thumb|Philamentous]] | ||
|[[Image:Newcastle_Hoppings_004.JPG|thumb]] | |[[Image:Newcastle_Hoppings_004.JPG|thumb]] | ||
Revision as of 13:46, 24 June 2010
Wednesday Morning
Cells are made competent ready for transformation.
- Positive control(plasmid uncut)- to make sure cells are transfroming properly
- Negative control (just cells)- nothing but competent cells
5 microfuge tubes:
- 1:5
- 1:3
- Vector
- Negative add nothing
- Positive add 1μl uncut
Wednesday afternoon taking a break
 |  |  |  |
 |  |  |  |
 |  |  |  |
 |  |  |  |
 |  |  |  |
