"

From 2010.igem.org

Revision as of 09:37, 24 June 2010 (view source) RachelBoyd (Talk | contribs) ← Older edit		Revision as of 11:13, 24 June 2010 (view source) Jannetta (Talk | contribs) (→Streak plating) Newer edit →
Line 12:		Line 12:
	* Flame tops of bottles lightly.		* Flame tops of bottles lightly.
	===Streak plating===		===Streak plating===
-	Deena's steak plate picture.	+	[[Image:Newcastle_Deena_streakplate.JPG|alt="|Star plate streaking student, Deena, shows off how it is to be done!"|Caption="Star plate streaking student, Deena, shows off how it is to be done!"]]
		+
		+	Star plate streaking student, Deena, shows off how it is to be done!
	===Set up culture for minipreps===		===Set up culture for minipreps===

---

Revision as of 11:13, 24 June 2010

Monday

Wet Lab techniques practice

Tips:

• Treat everything as a pathogen!
• Work around the bunsen burner.
• Use a control.
• Clean the bench at the end of the day!
• Heat the wire loop from the middle to the tip.
• Keep plates upside down.
• Keep lids off for as short a time as possible.
• Loosen all the tops off the vessels before you start.
• Flame tops of bottles lightly.

Streak plating

Star plate streaking student, Deena, shows off how it is to be done!

Set up culture for minipreps

5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.