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Team:Panama/28 September 2010
From 2010.igem.org
(New page: Digestion of Rh1ab with Pst1 -Electrophoresis Clone Rh1ab into the plasmid -This week PCR to show the RH1ab gene from within the plasmid it was cloned into Mutagenesis (Next week) Th...) |
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-This week | -This week | ||
| - | PCR to show the RH1ab gene | + | PCR to show the RH1ab gene |
Mutagenesis (Next week) | Mutagenesis (Next week) | ||
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1) We don't see the expected bands, possibly because we aren't using a purified PCR product | 1) We don't see the expected bands, possibly because we aren't using a purified PCR product | ||
| + | |||
2) We can't see the product. We will have to repeat the ligation | 2) We can't see the product. We will have to repeat the ligation | ||
| + | |||
3) We observe the band at the expected size | 3) We observe the band at the expected size | ||
| - | + | Measurement of the concentration of the purification: 96.1 ug/ml | |
Cloning Protocol of the pGEM-T Vector System | Cloning Protocol of the pGEM-T Vector System | ||
*Table | *Table | ||
Revision as of 20:58, 21 October 2010
Digestion of Rh1ab with Pst1
-Electrophoresis
Clone Rh1ab into the plasmid
-This week
PCR to show the RH1ab gene
Mutagenesis (Next week)
The gene was purified with the Roche Kit: High Pure PCR Product Purification Kit
The purification of the gene RH1ab was done because it is a requirement in order to be able to use with the pGEM-T Easy Vector system and to clone it into a vector to later do the mutagenesis
Run a gel to see the disgestion of the gene and to verify the cut sites of Pst1
- Table (p.61) and Picture (p.63)
1) We don't see the expected bands, possibly because we aren't using a purified PCR product
2) We can't see the product. We will have to repeat the ligation
3) We observe the band at the expected size
Measurement of the concentration of the purification: 96.1 ug/ml
Cloning Protocol of the pGEM-T Vector System
- Table
