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Team:DTU-Denmark
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<h3>Basic Concept</h3> | <h3>Basic Concept</h3> | ||
<table class="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" align="center"> | <table class="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" align="center"> | ||
| - | <caption align="bottom"><p align="justify"><b>Figure 2</b>: The basic concept of our project is illustrated:<br><i>Inducer 1</i> will activate <i>Promoter 1</i> thereby start up the production of <i>Repressor 1</i>, switching off the other part of our switch by repressing <i>Promoter 2</i>. This will essentially make the cells change from <font color="#990000">red</font color> to <font color="# | + | <caption align="bottom"><p align="justify"><b>Figure 2</b>: The basic concept of our project is illustrated:<br><i>Inducer 1</i> will activate <i>Promoter 1</i> thereby start up the production of <i>Repressor 1</i>, switching off the other part of our switch by repressing <i>Promoter 2</i>. This will essentially make the cells change from <font color="#990000">red</font color> to <font color="#600">green</font color>! This means that when the cells are exposed to <i>Inducer 2</i>, the cell culture will turn from <font color="#600">green</font color> to <font color="#990000">red</font color>.</font></p></caption> |
<tr><td><img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="400px"></td></tr> | <tr><td><img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="400px"></td></tr> | ||
</table><br> | </table><br> | ||
Revision as of 18:40, 20 October 2010
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DTU DENMARK The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems. We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks. Basic Concept
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Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event! Check out our active blog: now with picture of the day! Pictures have been posted so check out our picture gallery! |
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