Team:UNIPV-Pavia/Parts/Characterization
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(→Data analysis for RPU evaluation) |
(→Data analysis for RPU evaluation) |
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* phi is the considered promoter and J23101 is the reference standard promoter (taken from Anderson Promoter Collection); | * phi is the considered promoter and J23101 is the reference standard promoter (taken from Anderson Promoter Collection); | ||
* F is the blanked fluorescence of the culture, computed subtracting for each time sample fluorescence measure for negative control from that of culture, where the negative control is a non-fluorescent strain (in our experiment it is usually used TOP10 strain bearing BBa_B0032 or BBa_B0033, which are symmply RBSs do not have expression systems for reporter genes); | * F is the blanked fluorescence of the culture, computed subtracting for each time sample fluorescence measure for negative control from that of culture, where the negative control is a non-fluorescent strain (in our experiment it is usually used TOP10 strain bearing BBa_B0032 or BBa_B0033, which are symmply RBSs do not have expression systems for reporter genes); | ||
- | * ABS is the blanked absorbance (O.D.600) of the culture, computed as described in " | + | * ABS is the blanked absorbance (O.D.600) of the culture, computed as described in "Preliminary remarks" section. |
+ | RPU measurement has the following advantages (under suitable conditions) | ||
+ | * it is proportional to PoPS (Polymerase Per Second), a very important parameter that expresses the transcription rate of a promoter; | ||
+ | * it uses a reference standard and so measurements can be compared between different laboratories. | ||
Revision as of 15:52, 20 October 2010
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