"
Team:Aberdeen Scotland/Curve Fitting
From 2010.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
<p>Based on a graph in a paper by Witherell et al.<sup style="font-size:10px">[14]</sup> which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n<sub style="font-size:10px">1</sub>. Our two MS2 stem loops (see Fig 1 in <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Equations">Equations</a>) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).</p> | <p>Based on a graph in a paper by Witherell et al.<sup style="font-size:10px">[14]</sup> which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n<sub style="font-size:10px">1</sub>. Our two MS2 stem loops (see Fig 1 in <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Equations">Equations</a>) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).</p> | ||
| + | <br> | ||
<br> | <br> | ||
<center> | <center> | ||
| Line 12: | Line 13: | ||
<br> | <br> | ||
<center> | <center> | ||
| - | <img src="https://static.igem.org/mediawiki/2010/c/c4/Reproduced_graph.png" width=" | + | <img src="https://static.igem.org/mediawiki/2010/c/c4/Reproduced_graph.png" width="325" height="300"> |
</center> | </center> | ||
</html> | </html> | ||
Revision as of 13:48, 20 October 2010
University of Aberdeen - ayeSwitch
iGEM 2010
Curve Fitting to find the Hill Coefficient for the GFP/Bbox-stem Association (n2)
Based on a graph in a paper by Witherell et al.[14] which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n1. Our two MS2 stem loops (see Fig 1 in Equations) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).
