Team:UNIPV-Pavia/Parts/Characterization
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<html><p align="center"><font size="4"><b>CHARACTERIZATION</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>CHARACTERIZATION</b></font></p></html><hr><br> | ||
- | + | ==Growth conditions== | |
- | + | ===Microplate reader experiments for self-inducible promoters=== | |
* 8 ul of long term storage glycerol stock were inoculated in 1 ml of LB or M9 + suitable antibiotic in a 15 ml falcon tube and incubated at 37°C, 220 rpm for about 16 hours. | * 8 ul of long term storage glycerol stock were inoculated in 1 ml of LB or M9 + suitable antibiotic in a 15 ml falcon tube and incubated at 37°C, 220 rpm for about 16 hours. | ||
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** Variable experiment duration time (from 3 to 24 hours). | ** Variable experiment duration time (from 3 to 24 hours). | ||
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+ | ===Microplate reader experiments for constitutive promoters (R.P.U. evaluation)=== | ||
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+ | ===Data analysis for self-inducible promoters (initiation-treshold determination)=== | ||
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+ | ===Data analysis for RPU evaluation=== | ||
Revision as of 13:38, 20 October 2010
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