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Team:Aberdeen Scotland/Curve Fitting
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| - | <h1>Curve Fitting to find the Hill Coefficient | + | <h1>Curve Fitting to find the Hill Coefficient for the GFP/Bbox-stem Association (n<sub style="font-size:15px">2</sub>)</h1> |
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| + | <p>Based on a graph in a paper by Witherell et al.<sup style="font-size:15px">[14]</sup> which showed the binding curves of the MS2 stem loop we could calculate the value for n<sub style="font-size:15px">2</sub>. Our two MS2 stem loops (see Fig 1 in Equations) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).</p> | ||
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Revision as of 10:31, 20 October 2010
University of Aberdeen - ayeSwitch
iGEM 2010
Curve Fitting to find the Hill Coefficient for the GFP/Bbox-stem Association (n2)
Based on a graph in a paper by Witherell et al.[14] which showed the binding curves of the MS2 stem loop we could calculate the value for n2. Our two MS2 stem loops (see Fig 1 in Equations) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).
