Team:Wisconsin-Madison/experiments

From 2010.igem.org

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See entire procedure : [[Media:UW_colonicacidassay.pdf Download]]  
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See entire procedure : [[Media:UW_colonicacidassay.pdf]]  
See original reference: [[http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link]]
See original reference: [[http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link]]

Revision as of 03:11, 20 October 2010

Enzyme Treatment

Encapsulation

Colonic Acid Quantification

Parts used in this experiment
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG 500
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG 501
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG 502 <partinfo>BBa_k200021</partinfo> Empty vector - control Inducible - IPTG



See entire procedure : Media:UW_colonicacidassay.pdf See original reference: http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link

Cell Survivability Testing

Parts used in this experiment
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG 500
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG 501
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG 502

Best Combination


Inducible-Repressible Expression

Characterize pH Promoters

Parts Used: <partinfo>BBa_k318513</partinfo>

Amount of Regulators

IR-System - Arabinose

Parts Used: <partinfo>BBa_k318509</partinfo>, <partinfo>BBa_k318510</partinfo>, <partinfo>BBa_K318511</partinfo>, <partinfo>BBa_K318506</partinfo>

IR-System - pH

Parts Used: <partinfo>BBa_TUNED Part</partinfo>, <partinfo>BBa_K318506</partinfo>

IR-Lysis - pH

Parts Used: <partinfo>BBa_TUNED Part</partinfo>, <partinfo>BBa_K318507</partinfo>

Bile Induction

<partinfo>BBa_K318516</partinfo>, <partinfo>BBa_Lysis Part</partinfo> <partinfo>BBa_K318516</partinfo>, <partinfo>BBa_LYSIS Part</partinfo>

Encryption

Laboratory Notebooks

Media:Wisconsin-Madison2010_Notebook1.pdf

Media:Wisconsin-Madison2010_Notebook2.pdf

Media:Wisconsin-Madison2010_Notebook3.pdf