Team:Wisconsin-Madison/experiments
From 2010.igem.org
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- | See entire procedure : [[Media:UW_colonicacidassay.pdf | + | See entire procedure : [[Media:UW_colonicacidassay.pdf]] |
See original reference: [[http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link]] | See original reference: [[http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link]] | ||
Revision as of 03:11, 20 October 2010
Enzyme Treatment
Encapsulation
Colonic Acid Quantification
Part Number | Function | Expression Type | Zip File | |||
<partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 | |||
<partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 | |||
<partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 | <partinfo>BBa_k200021</partinfo> | Empty vector - control | Inducible - IPTG |
See entire procedure : Media:UW_colonicacidassay.pdf
See original reference: http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link
Cell Survivability Testing
Part Number | Function | Expression Type | Zip File |
<partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 |
<partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 |
<partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 |
Best Combination
Inducible-Repressible Expression
Characterize pH Promoters
Parts Used: <partinfo>BBa_k318513</partinfo>
Amount of Regulators
IR-System - Arabinose
Parts Used: <partinfo>BBa_k318509</partinfo>, <partinfo>BBa_k318510</partinfo>, <partinfo>BBa_K318511</partinfo>, <partinfo>BBa_K318506</partinfo>
IR-System - pH
Parts Used: <partinfo>BBa_TUNED Part</partinfo>, <partinfo>BBa_K318506</partinfo>
IR-Lysis - pH
Parts Used: <partinfo>BBa_TUNED Part</partinfo>, <partinfo>BBa_K318507</partinfo>
Bile Induction
<partinfo>BBa_K318516</partinfo>, <partinfo>BBa_Lysis Part</partinfo>
<partinfo>BBa_K318516</partinfo>, <partinfo>BBa_LYSIS Part</partinfo>
Encryption
Laboratory Notebooks
Media:Wisconsin-Madison2010_Notebook1.pdf
Media:Wisconsin-Madison2010_Notebook2.pdf
Media:Wisconsin-Madison2010_Notebook3.pdf