UTDallas/15 October 2010
From 2010.igem.org
(Difference between revisions)
(New page: *Miniprepped PyeaR+GFP and PyeaR+RFP. *Digested PyeaR+GFP, PyeaR+RFP, and pSB1C3, then ran a gel electrophoresis and gel purified. All of them were correct and gave good yields. *Ligated P...) |
|||
Line 4: | Line 4: | ||
[[Image:10-15.JPG | 500px | center]] | [[Image:10-15.JPG | 500px | center]] | ||
+ | |||
+ | Alkane Sensor | ||
+ | *Ligated dephosphorylated pSB1C3 to phosphorylated PalkB |
Revision as of 23:59, 19 October 2010
- Miniprepped PyeaR+GFP and PyeaR+RFP.
- Digested PyeaR+GFP, PyeaR+RFP, and pSB1C3, then ran a gel electrophoresis and gel purified. All of them were correct and gave good yields.
- Ligated PyeaR+GFP and PyeaR+RFP to pSB1C3, overnight.
Alkane Sensor
- Ligated dephosphorylated pSB1C3 to phosphorylated PalkB