Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/10

From 2010.igem.org

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(3:DNA Digestion)
(4:DNA Ligation)
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<Sample><br />
<Sample><br />
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*1-2   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]  
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*10-2  
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
(after digestion)
(after digestion)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
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===5:DNA extraction===
===5:DNA extraction===

Revision as of 12:07, 19 October 2010


Contents

2010/10/10

1:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/10)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-10 11hr 40min.JPG

<Result>
Matsuura 2010-10-10 21hr 24min.JPG

<Result>
Matsuura 2010-10-10 18hr 40min.JPG

<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG


2:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 1

3:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions


<Protocol>
See Prptpcpl 9

4:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 10

5:DNA extraction

<Member>
Mariko,mio,nito

<Sample>
PCR products

  • 2
  • 4
  • 8
  • 10
  • 5

<Protocol>
See Protocol 4


6:Plasmid extraction from E-coli

<Member>
nito

<Sample>

  • 12
  • 13
  • 15
  • 16

<Protocol>
See Protocol 6