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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/10
From 2010.igem.org
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *9 (60) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (60) |
| - | *1-2 (60) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] (60) |
| - | *2-10 (60) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] (60) |
| - | *7-8-10 (60) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] (60) |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
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===3：DNA Digestion=== | ===3：DNA Digestion=== | ||
Revision as of 12:06, 19 October 2010
Contents |
2010/10/10
1:Electrophoresis
<Member>
nito
<Sample>
PCR products (10/10)
<Protocol>
See Protocol 8
<Result>
<Result>
<Result>
<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG
2:PCR
<Member>
nito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
3：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- 5
・Digest enzyme
- Spe1
＜Protocol＞
See Prptpcpl 9
4：DNA Ligation
＜Member＞
nito
＜Sample＞
- 1-2
- 10-2
(after digestion)
＜Protocol＞
See Protocol 10
5：DNA extraction
＜Member＞
Mariko,mio,nito
＜Sample＞
PCR products
- 2
- 4
- 8
- 10
- 5
＜Protocol＞
See Protocol 4
6:Plasmid extraction from E-coli
<Member>
nito
<Sample>
- 12
- 13
- 15
- 16
<Protocol>
See Protocol 6
