Team:Michigan/Virus Surface Display
From 2010.igem.org
(Difference between revisions)
Line 69: | Line 69: | ||
• aliquot INP samples and primers for sequencing | • aliquot INP samples and primers for sequencing | ||
+ | 7/26/2010 | ||
+ | • Electroporation transformation of the following Biobrick parts: | ||
+ | pBad/araC (I0500) | ||
+ | Linker (K157013) | ||
+ | GFP (E0040) | ||
7/27/2010 | 7/27/2010 | ||
Line 115: | Line 120: | ||
10:00 | 10:00 | ||
- | • | + | • Ligation of digested Biobrick parts: |
- | + | - INP + Linker | |
- | + | - OmpA + GFP | |
- | + | - INP + GFP | |
- | + | • Heat shock transformation of ligation | |
- | + | ||
- | • | + | |
+ | 8/2/2010 | ||
+ | • Miniprep of INP-linker | ||
+ | • Nanodrop results: | ||
+ | INP + Linker construct ng/uL OD260/280 OD260/230 | ||
+ | 1.1 20.7 1.88 1.73 | ||
+ | 1.2 61.4 2.04 2.09 | ||
+ | 2.1 59.8 1.99 2.06 | ||
+ | 2.2 69.8 2.02 2.11 | ||
|width="250px" style="background: transparent"| | |width="250px" style="background: transparent"| | ||
Revision as of 06:48, 17 October 2010