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Team:Calgary/18 June 2010
From 2010.igem.org
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'''Friday June 18, 2010''' | '''Friday June 18, 2010''' | ||
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| + | [[Image:06.17.2010_B0015+R0040_PCRGel.png|thumb|400px|Jeremy's gel electrophoresis of the colonies resulting from the construction of B0015 (double terminators) and R0040 (TetR repressible promoter)]] | ||
Emily: Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes. The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part. Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday. | Emily: Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes. The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part. Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday. | ||
Dev, Jeremy, and Chris: | Dev, Jeremy, and Chris: | ||
Revision as of 15:29, 21 June 2010
Friday June 18, 2010
Emily: Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes. The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part. Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday.
Dev, Jeremy, and Chris:
