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Week13 9/5/10-9/11/10
From 2010.igem.org
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| + | ===Week12 Summary=== | ||
| + | We began reorganizing our lab space and equipment in preparation for a fall quarter class that would be utilizing the majority of the lab. We attempted standard assembly of LacP-RBS-CHS3 because the presence of a P site in CHS3 prevented 3A assembly. | ||
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===9/6/10=== | ===9/6/10=== | ||
Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room. | Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room. | ||
Revision as of 22:42, 16 October 2010
Week12 Summary
We began reorganizing our lab space and equipment in preparation for a fall quarter class that would be utilizing the majority of the lab. We attempted standard assembly of LacP-RBS-CHS3 because the presence of a P site in CHS3 prevented 3A assembly.
9/6/10
Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room.
9/7/10
9/8/10
9/9/10
Assembled LacP-RBS1-CHS3 in a Chlor backbone plasmid using standard assembly methods Cut LacP-RBS1 using S Cut CHS3 using X and S (50% chance of inserting backwards --> must select for this after)
9/10/10
Inoculated 20 LacP-RBS-CHS3 cells.
Assembled CP-LacPI-RFP parts into Tet backbone.
