"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
(→7：DNA Digestion) |
(→8：DNA Ligation) |
||
| Line 102: | Line 102: | ||
===8：DNA Ligation === | ===8：DNA Ligation === | ||
＜Member＞<br /> | ＜Member＞<br /> | ||
| - | + | Hitomi | |
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | * | + | *1-2-3 |
| - | * | + | *4-5 |
| - | * | + | *6-7 |
| - | * | + | *9-7 |
| - | + | ||
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | ||
| - | |||
===9:PCR=== | ===9:PCR=== | ||
Revision as of 11:39, 16 October 2010
Contents |
2010/10/15
1：DNA extraction
＜Member＞
Hitomi
＜Sample＞
- 4-5
- 9-7
- vector
＜Protocol＞
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
- 4-5
- 9-7
<Protocol>
See Protocol 8
<Result>
3：DNA extraction from gels
＜Member＞
Mariko
＜Sample＞
- 4-5
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
- vector
- 4-5
- 9-7
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
- 4-5
- 9-7
- 9
- vector
<Protocol>
See Protocol 8
<Result>
6:PCR
<Member>
mio
<Sample>
- 9
<Protocol>
See Protocol 2
7：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
- vector
- 9-7
＜Protocol＞
See Protocol 9
8：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
- 1-2-3
- 4-5
- 6-7
- 9-7
＜Protocol＞
See Protocol 3
9:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
10:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
12:Transformation
<Member>
nito
<Sample>
- 7-8-10
- JM109
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
14:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
